Main description:

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.

Contents:

Extraction/Characterization of DNA
Immunoprecipitation for Isolation of Protein Signaling Complexes from Native Tissues
Realtime PCR
Single Cell Gel Electrophoresis
High Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
Difference Gel Electrophoresis
Optimization of Cell Cycle Measurement by Time Lapse-Microscopy
Transfection
Cell Enrichment
Magnetic Batch Separation
Lateral Flow Assays
Measurement of Cell Regeneration
DNA Damage Analysis in Cells
Purification of Cardiac Cells
Endothelial Cell Tube Formation in vitro assays
Single-particle Analysis
Measurement of Cell Invasivness
Techniques for Cell Sorting
Stem Cells for Drug Discovery
Quantifying Apoptosis in 96-well Plates
Pulse Cytometry
Cell Motility
Single Cell Deposition
Enzyme Sensors for Living Cells
Myoblast purifying by magnetic-activated cell sorting (MACS)