Main description:

Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more.

Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms.

Contents:

About the Author Preface Acknowledgements Introduction to the Student

Section I: Microbiological Media and Cultural Techniques

1. Preparation of Media and Sterilization Techniques 2. Microorganisms in Environment 3. Aseptic Techniques 4. Pure Culture Techniques 5. Culture Preservation Techniques

Section II: Techniques on Morphological Examination of Bacteria

6. Microscope for Study of Microorganisms 7. Microbial Cell Measurement Technique 8. Wet-mount and Hanging-drop Techniques 9. Smear Preparation and Simple Staining Techniques 10. Gram Staining Technique 11. Bacterial Endospore Staining Techniques 12. Bacterial Capsule Staining Techniques 13. Motility Tests

Section III: Biochemical and Cultural Characteristics of Microorganisms

14. Cultural Characteristics of Bacteria 15. Oxidation and Fermentation 16. Hydrolytic Reactions

Section IV: Chemical and Physical Effects on Microorganisms

17. Antiseptic and Disinfectant Effects on Microorganisms 18. Antibiotic Sensitivity Test Technique 19. Effect of Hand Scrubbing on Removal of Microorganisms 20. Temperature Effects on Microorganisms 21. Osmotic Pressure and pH Effects on Microorganisms 22. Ultraviolet Light Effects on Microorganisms 23. Oxygen Effects on Microorganisms

Section V: Fungi and Bacteriophages

24. Morphological Characteristics of Yeasts and Molds 25. Isolation, Enumeration, and Uses of Bacteriophages

Appendix Bibliography Index