Main description:

This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusing on advances in technology, alternate matrices, and rapidly-changing classes of drugs of abuse, compounds pertinent to toxicology, and therapeutic agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, LC-MS in Drug Analysis: Methods and Protocols, Second Edition aims to ensure successful reults in the further study of this vital field.

Contents:

1. An Introduction to Drug Testing - The Expanding Role of Mass Spectrometry

Christine L.H. Snozek, Loralie J. Langman, and Steven W. Cotten

2. Quantification of Eight Cannabinoids Including Cannabidiol in Human Urine Via Liquid Chromatography Tandem Mass Spectrometry

Karl B. Scheidweiler and Allan J. Barnes

3. Analysis of Benzodiazepines for Drug Facilitated Assaults and Abuse Settings (Urine)

Olaf H. Drummer, Matthew Di Rago, and Dimitri Gerostamoulos

4. Targeted Opioid Screening Assay for Pain Management Using High Resolution Mass Spectrometry

Darlington Danso, Loralie J. Langman, and Paul J. Jannetto

5. Measurement of Buprenorphine and Norbuprenorphine in Urine

Andrea R. Terrell, Vipin Adhlakha, and Poluru Reddy

6. Quantitation of Tapentadol by Liquid Chromatography - Tandem Mass Spectrometry

Graham R. Jones and Russell P. Handy

7. Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS

He Sarina Yang and Leslie Edinboro

8. LC-MS/MS method for the quantification of the Leflunomide metabolite, Teriflunomide, in Human Serum/Plasma

Geoffrey S. Rule, Alan L. Rockwood, and Kamisha L. Johnson-Davis

9. Analysis of Tryptic Peptides from Therapeutic Monoclonal Antibodies using LC-MS/MS

Maria Alice Vieira Willrich

10. Quantification of Methotrexate in Human Serum and Plasma by Liquid Chromatography Tandem Mass Spectrometry

Gabrielle N. Winston-McPherson, Michael Schmeling, and Andrew N. Hoofnagle

11. Simultaneous Determination of Tacrolimus and Cyclosporine A in Whole Blood by Ultra-Fast LC-MS/MS

Matthew W Bjergum, Paul J Jannetto, and Loralie J Langman,

12. Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) Method to Quantify Gabapentin and Pregabalin in Urine

Stephen Merrigan and Kamisha L. Johnson-Davis1

13. The Evolving Landscape of Designer Drugs

Sherri L. Kacinko and Donna M. Papsun

14. Analysis of Synthetic Cannabinoid Metabolites by Liquid Chromatography-Tandem Mass Spectrometry

Gregory C. Janis

15. Quantification of Designer Opioids by Liquid Chromatography Tandem Mass Spectrometry

Sherri L. Kacinko and Joseph W. Homan

16. Screening Analysis for Designer Stimulants by LC-MS/MS

Piotr Adamowicz and Bogdan Tokarczyk

17. Drug Screening Using Liquid Chromatography Quadrupole Time of Flight (LC-QqTOF) Mass Spectrometry

Jennifer M. Colby and Kara L. Lynch

18. Alternate Matrices - Meconium, Cord Tissue, Hair, and Oral Fluid

Kendra L. Palmer and Matthew D. Krasowski

19. Salt-assisted Liquid-Liquid Extraction of Meconium for Analysis of Cocaine and Amphetamines by Liquid Chromatography-tandem Mass Spectrometry

Melissa M. Goggin and Gregory C. Janis

20. Detection of In Utero Cannabis Exposure in Umbilical Cord Tissue by A Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method

Fang Wu, Triniti L. Scroggin, and Gwendolyn A. McMillin

21. Quantitation of Ethyl -D-glucuronide in Human Umbilical Cord Tissue by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Simuli L. Wabuyele and Gwendolyn A. McMillin

22. Analysis of Drugs in Oral Fluid using LC-MS/MS

Cynthia Coulter and Christine Moore

23. Determination of Cocaine and Metabolites in Dried Blood Spots by LC-MS/MS

Lars Ambach and Christophe Stove