Main description:

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

Contents:

1. The Model Legume Genomes

Steven B. Cannon

2. Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization

Rene Geurts and Hans de Jong

3. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes

Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas, and Robert M. Stupar

4. RNA-Seq for Transcriptome Analysis in Non-Model Plants

Rohini Garg and Mukesh Jain

5. Functional Analysis of Legume Genome Arrays

Nicolas Goffard and Georg Weiller

6. Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing

Tian-Zuo Wang and Wen-Hao Zhang

7. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes

Cecilia Contreras-Cubas, Alejandra A. Covarrubias, and Jose Luis Reyes

8. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines

Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, and Kirankumar S. Mysore

9. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection

Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu , Ilaria Losini, Pietro Piffanelli, Sergio Arcioni, and Carla Scotti

10. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants

Dorian Fabian Urbanski, Anna Malolepszy, Jens Stougaard, and Stig Uggerhoj Andersen

11. Isolation and Forward Genetic Analysis of Developmental Genes in Pea

James L. Weller, Valerie F.G. Hecht, and Frances C. Sussmilch

12. Gene Silencing in Medicago truncatula Roots Using RNAi

Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, and Maria J. Harrison

13. Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)

Islam Shahidul, Huaan Yang, and Guijun Yan

14. Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens

Youhong Song, Kim E. Nolan, and Ray J. Rose

15. Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration

Kelsey Picard, Robyn Lee, Roger Hellens, and Richard C. Macknight

16. Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins

David A. Collings

17. Proteomics and the Analysis of Nodulation

Nijat Imin

18. Phosphoproteomic Analysis of Peptides

Mark A. Baker

19. Plant Metabolomics: From Experimental Design to Knowledge Extraction

Amit Rai, Shivshankar Umashankar, and Sanjay Swarup