Main description:

In Leukemia and Lymphoma: Detection of Minimal Residual Disease, hands-on experts describe and discuss the minimal residual disease (MRD) methods they have successfully pioneered for leukemias and lymphomas. They apply reverse transcription PCR (RT-PCR) to acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), and acute promyelocytic leukemia (APL). Other PCR methods are used for Non-Hodgkin's Lymphoma and for the monitoring of follicular lymphoma. Additional chapters address the use of real-time quantitative PCR (RQ-PCR), the emergent method of choice, in patients with acute lymphoblastic leukemia (ALL), the evaluation of MRD techniques in clinical trials, and the application of flow cytometry techniques.

Contents:

Flow-Cytometry Methods for the Detection of Residual Leukemia
Richard Ratei and Wolf-Dieter Ludwig

Flow-Cytometry-Based Studies of Minimal Residual Disease in Children with Acute Lymphoblastic Leukemia
Dario Campana

PCR Methods for the Detection of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia
Geoffrey Neale

Statistical Considerations in the Analysis of Minimal Residual Disease
Dennis A. Johnston and Theodore F. Zipf

The Application of Minimal Residual Disease Detection Methodology to the Clinical Decision-Making Process
Theodore F. Zipf and Dennis A. Johnston

Measurement of Minimal Residual Disease in Children Undergoing Allogeneic Stem Cell Transplant for Acute Lymphoblastic Leukemia
John Moppett, Amos Burke, Christopher Knechtli, Anthony Oakhill, Colin Steward, and Nicholas J. Goulden

Molecular Investigation of Minimal Residual Disease in Adult Acute Lymphoblastic Leukemia of B Lineage
Letizia Foroni, Forida Y. Mortuza, and A. Victor Hoffbrand

Investigation of Minimal Residual Disease in Acute Myeloid Leukemia by Immunophenotyping
Jesus F. San Miguel, Maria B. Vidriales, and Alberto Orfao

Minimal Residual Disease in Acute Myeloid Leukemia: RT-PCR-Based Studies of Fusion Transcripts
Guido Marcucci and Michael A. Caligiuri

Minimal Residual Disease in Acute Promyelocytic Leukemia
Francesco Lo Coco and Daniela Diverio

Minimal Residual Disease in Chronic Myelogenous Leukemia: Results of RT-PCR Detection of BCR-ABL Transcripts
Nicholas C. P. Cross and Andreas Hochhaus

Utility of PCR Assessment of Minimal Residual Disease After Stem Cell Transplantation in Non-Hodgkin's Lymphoma
Angela M. Krackhardt and John G. Gribben

Real-Time Polymerase Chain Reaction of Genomic DNA for Quantitation of t(14;18)
Apostolia-Marie Tsimberidou, Yunfang Jiang, Richard J.Ford, Fernando Cabanillas, and Andreas H. Sarris

Monitoring Follicular Lymphoma by Polymerase Chain Reaction
Ming-Sheng Lee and Fernando Cabanillas

Editors' Notes

Index