Main description:

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

Contents:

Part I: Introductory Reviews

1. Introduction and Classification of Leukemias

Geoffrey Brown

2. Leukemia Stem Cells: Concept and Implications

Isidro Sanchez-Garcia and Cesar Cobaleda

3. Leukemia Stem Cell Drug Discovery

Cesar Cobaleda and Isidro Sanchez-Garcia

Part II: Protocols

4. Analysis and Isolation of Mouse Leukemic Stem Cells

Fang Dong, Haitao Bai, and Hideo Ema

5. Mass Cytometry of Hematopoietic Cells

Astraea Jager, Jolanda Sarno, and Kara Davis

6. PCR Technology to Identify Minimal Residual Disease

Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group

7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies

Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Bruggemann, on behalf of the EuroClonality-NGS Working Group

8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

Daniel Hein, Arndt Borkhardt, and Ute Fischer

9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells

Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic

10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly

11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation

Kyomi J. Igarashi and Ryo Yamamoto

12. Experimental Competitive Bone Marrow Transplant Assays

Roxann Hetu-Arbour, Sarah Bouali, and Krista M. Heinonen

13. Human T-ALL Xenografts

Patricia Fuentes, Maria L. Toribio, and Sara Gonzalez-Garcia

14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts

Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci

15. Leukemic Stem Cell Culture in Cytokine-Free Medium

Xiaolei Liu and Peter S. Klein

16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman

17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

Cristina Prieto, Damia Romero-Moya, and Rosa Montes

18. Lentiviral Transduction for Optimal LSC/HSC Manipulation

Gustavo Mostoslavsky

19. Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes

Yu Wei Zhang, Julian Mess, and Nina Cabezas-Wallscheid

20. Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes

Sabrina Jacobs, Leonid V. Bystrykh, and Mirjam E. Belderbos

21. Arrayed Molecular Barcoding of Leukemic Stem Cells

Marion Chapellier and Marcus Jaras

22. In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis

Carolina Vicente-Duenas

23. In Situ Hematopoietic Stem Cell Imaging

Aparna Venkatraman, Sarah E. Smith, Sandra Pinho, Meng Zhao, Linheng Li, and Paul Frenette

24. A Genome-Editing System for Therapeutical Targeting of Stem Cells

Giacomo Frati and Annarita Miccio

25. Method for the Generation of Induced Hematopoietic Stem Cells

Leonid Olender, Klil Levy, and Roi Gazit

26. Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells

Andre G. Deslauriers, Andriana G. Kotini, and Eirini P. Papapetrou

27. High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells

Lorenzo Veschini, Heba Sailem, Disha Malani, Vilja Pietiainen, Ana Stojiljkovic, Erika Wiseman, and Davide Danovi

28. Bioinformatic Methods to Identify Mutational Signatures in Cancer

S.M. Ashiqul Islam and Ludmil B. Alexandrov