Main description:

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

Contents:

Part I: Overview of Lipases, Phospholipases and Related Enzymes

1. Lipases: An Overview Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty

2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham

3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus Monica Sanchez-Gonzalez, Allan Blanco-Gamez, Roberto Parra-Saldivar, Juan Carlos Mateos-Diaz and Maria Isabel Estrada Alvarado

4. Phospholipases: An Overview Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri and Michael H. Gelb

Part II: Screening and Sources of Lipases, Phospholipases and Its Inhibitors

5. High-Throughput Sceening Method for Lipases/Esterases Eduardo Mateos-Diaz, Jorge Alberto Rodriguez, Maria de los Angeles Camacho-Ruiz, Juan Carlos Mateos-Diaz

6. Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries

Dolores Reyes-Duarte, Manuel Ferrer, and Humberto Garcia-Arellano

7. Plant Lipases:Partial Purification of C. papaya Lipase

Ivanna Rivera, Juan Carlos Mateos-Diaz, and Georgina Sandoval

8. Plant Phospholipases: an Overview

Geliang Wang, Stephen Ryu, and Xuemin Wang

9. Lipase and Phospholipase Inhibitors: Design and Applications

Mark E. Venable

10. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors

Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing and Michael H. Gelb

Part III Cloning, Production, Purification and Characterization of Lipases and Phospholipases

11. Heterologous Expression Systems for Lipases: A Review

Francisco Valero

12. Streptomyces Phospholipase D: Cloning and Production

Yozo Nakazawa

13. Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses

Julian Gomez-Cambronero and Karen M. Henkels

14. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite

Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres

15. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27

Pablo Fucinos, Ramon Gonzalez, Estrella Atanes, Ana Belem Fernandez Sestelo, Nelson Perez-Guerra, Lorenzo Pastrana and Maria Luisa Rua

16. Purification, Biochemical and Kinetic Properties of Recombinant
Staphylococcus aureus Lipase

Habib Horchani, Ahmed Fendri, Hanen Louatia, Adel Sayari, Youssef Gargouri and Robert Verger

17. Phospholipase A2 Purification and Characterization; A Case Study

Aida Karray, Youssef Gargouri, Robert Verger and Sofiane Bezzine

Part IV: Optimization of the Biocatalyst and Reaction Conditions

18. The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases

Sophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud and Alain Martya

19. Molecular Modeling of Lipase Binding to a Substrate-Water Interface

Christian C. Gruber and Jurgen Pleiss

20. Different Strategies for Hyperactivation of Lipase Biocatalysts

Jose M. Palomo and Jose M. Guisan

21. Rational Design of Immobilized Lipases and Phospholipases

Alberto del Monte Martinez and Bessy V. Cutino Avila

22. Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions

Edmundo Castillo, Alejandro Torres-Gavilan, Georgina Sandoval , Alain Marty

Part V: Applications of Lipases and Phopholipases

23. Lipases as Biocatalysts for the Synthesis of Structured Lipids Ram Chandra Reddy Jala, Peng Hu, Tiankui Yang, Yuanrong Jiang, Yan Zheng, Xuebing Xu

24. Lipases-Catalyzed Modification of Phenolic Antioxidants Pamela Torres, Dolores Reyes-Duarte, Antonio Ballesteros and Francisco J. Plou

25. Lipases as Catalysts in Synthesis of Fine Chemicals Alicia Baldessari

26. Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides Alicia Baldessari and Luis E. Iglesias

27. Lipases as Biocatalyst for Biodiesel Production Xiaohu Fan, Xochitl Niheus, Georgina Sandoval

28. Biopolymer Synthesis Catalyzed by Lipases Karla A. Barrera-Rivera, Arturo Flores-Carreon, Antonio Martinez-Richa

29. Phospholipases in Food Industry-A Review Victor Casado, Diana Martin, Carlos Torres, Guillermo Reglero

30. Lipase and Phospholipase Biosensors: A Review Enrique J. Herrera-Lopez