Main description:

Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.

Contents:

1. Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions

Soren B. Nielsen and Daniel E. Otzen

2. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipid Membranes

Vesna Hodnik and Gregor Anderluh

3. Probing the Thermodynamics of Protein-Lipid Interactions by Isothermal Titration Calorimetry

Musti J. Swamy and Rajeshwer S. Sankhala

4. Differential Scanning Calorimetry of Protein-Lipid Interactions

Olga Canadas and Cristina Casals

5. Atomic Force Microscopy and Spectroscopy to Probe Single Membrane Proteins in Lipid Bilayers

K. Tanuj Sapra

6. Optimized Negative-Staining Protocol for Lipid-Protein Interactions Investigated by Electron Microscopy

Mark Garewal, Lei Zhang, and Gang Ren

7. Examining the Protein-Lipid Complexes using Neutron Scattering

Luke A. Clifton, Cameron Neylon, and Jeremy H. Lakey

8. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

Koichi Matsuo and Kunihiko Gekko

9. Structural Characterization of Membrane Proteins and Peptides by FTIR and ATR-FTIR Spectroscopy

Suren A. Tatulian

10. Foerster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity

Luis M. S. Loura, Manuel Prieto, and Fabio Fernandes

11. A Guide to Tracking Single Transmembrane Proteins in Supported Lipid Bilayers

Kumud Raj Poudel, Jeffrey P. Jones, and James A. Brozik

12. Fluorescence Correlation Spectroscopy to Examine Protein Lipid Interactions in Membranes

Viktoria Betaneli and Petra Schwille

13. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching

Gregory A. Caputo and Erwin London

14. Studying Lipid-Protein Interactions with Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids

Tibor Pali and Zoltan Kota

15. EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers

Enrica Bordignon and Yevhen Polyhach

16. Solid-State NMR Approaches to Study Protein Structure and Protein-Lipid Interactions

Christopher Aisenbrey, Matthias Michalek, Evgeniy S. Salnikov and Burkhard Bechinger

17. Solution NMR Spectroscopy for the Determination of Structures of Membrane Proteins in a Lipid Environment

Ashish Arora

18. Nanodiscs as a New Tool to Examine Lipid-Protein Interactions

Mary A. Schuler, Ilia G. Denisov, and Stephen G. Sligar

19. The Simulation Approach to Lipid-Protein Interactions

Teresa Paramo, Diana Garzon, Daniel A. Holdbrook, Syma Khalid, and Peter J. Bond