Main description:

This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes. Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility); derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.

Contents:

1. Introduction and Overview of Lipidomic Strategies

William J. Griffiths and Yuqin Wang

2. Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry

Paul L. Wood

3. Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Mark R. Emmett and Cheryl F. Lichti

4. MALDI-Imaging Mass Spectrometry of Brain Lipids

Norelle C. Wildburger

5. Ion-Mobility Mass Spectrometry for Lipidomics Applications

Giuseppe Paglia, Bindesh Shrestha, and Giuseppe Astarita

6. In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteins: A Lesson from the Ceramide Transport Protein CERT

Kentaro Hanada and Toshihiko Sugiki

7. Quantification of Endogenous Endocannabinoids by LC-MS/MS

Mesut Bilgin and Andrej Shevchenko

8. Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry

Takayuki Yamada and Takeshi Bamba

9. Mass Spectrometric Analysis of Lipid Hydroperoxides

Tania Melo, Elisabete Maciel, Ana Reis, Pedro Domingues, and M. Rosario M. Domingues

10. Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradation of (2E)-hexadecenal in HepG2 Cell Lysates

Corinna Neuber, Fabian Schumacher, Erich Gulbins, and Burkhard Kleuser

11. CE Analysis of Phospholipid Headgroups Vaclav Matej Bierhanzl, Martina Riesova, Gabriela Seydlova and Radomir Cabala

12. HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids

Tatiana Kondakova, Nadine Merlet Machour, and Cecile Duclairoir Poc

13. Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures

Candice Z. Ulmer, Richard A. Yost, and Timothy J. Garrett

14. Ambient Lipidomic Analysis of Brain Tissue using Desorption Electrospray Ionization (DESI) Mass Spectrometry

Valentina Pirro, Alan K. Jarmusch, Christina R. Ferreira, and R. Graham Cooks

15. Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids Alexandra C Kendall and Anna Nicolaou

16. Derivatization of Fatty Aldehydes and Ketones: Girard's Reagent T (GRT)

Paul L. Wood

17. Derivatization of Lipid Amines: Fluorenylmethyloxycarbonyl (FMOC) Paul L. Wood

18. High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products Paul L. Wood