Main description:

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Liposomes Came First: The Early History of Liposomology
Volkmar Weissig

2. Thin Film Hydration followed by Extrusion Method for Liposome Preparation
Hongwei Zhang

3. Preparation of DRV Liposomes
Sophia G. Antimisiaris

4. Method of Simultaneous Analysis of Liposome Components by HPTLC/FID
Sophia Hatziantoniou and Costas Demetzos

5. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Brigitte Papahadjopoulos-Sternberg

6. Liposome Formulations of Hydrophobic Drugs
Reto A. Schwendener and Herbert Schott

7. A "Dock and Lock" Approach to Preparation of Targeted Liposomes
Marina V. Backer and Joseph M. Backer

8. Coupling of Ligands to the Liposome Surface by Click Chemistry
Maria Vittoria Spanedda, Marcella De Giorgi, Fatouma Said Hassane, Francis Schuber, Line Bourel-Bonnet, and Benoit Frisch

9. Elastic Liposomes for Topical and Transdermal Drug Delivery
Melani A. Solomon

11. Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Yvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, and Anil Vangala

12. Liposome Biodistribution via Europium Complexes
Nathalie Mignet and Daniel Scherman

13. Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy<
Beth Goins, Ande Bao, and William T. Phillips

14. Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes
Suna Erdogan and Vladimir P. Torchilin

15. Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes
Nicolas Bertrand, Pierre Simard, and Jean-Christophe Leroux

16. Long-Circulating, pH-Sensitive Liposomes
Denitsa Momekova, Stanislav Rangelov, and Nikolay Lambov

17. Anionic pH-Sensitive Lipoplexes
Nathalie Mignet and Daniel Scherman

18. Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Edwin Pozharski

19. Preparation and Physical Characterization of DNA Binding Cationic Liposomes
Vaibhav Saxena

20. Fluorescence Resonance Energy Transfer (FRET) Based Analysis of Lipoplexes
Edwin Pozharski

21. Targeted Magnetic Liposomes Loaded with Doxorubicin
Pallab Pradhan, Rinti Banerjee, Dhirendra Bahadur, Christian Koch, Olga Mykhaylyk, and Christian Plank

22. Stable Discoidal Bicelles - A Platform of Lipid Nanocarriers for Cellular Delivery
Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh and Mu-Ping Nieh