Main description:

This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and characterization of glycopolymers; glycopolymer-nanoparticle conjugates; and surface immobilized glycopolymers. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Macro-Glycoligands: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Part I: Synthesis and Characterization of Glycopolymers

1. Synthesis of Chain-end Functionalized Glycopolymers via Cyanoxyl Mediated Free Radical Polymerization (CMFRP)

Valentinas Gruzdys, Jinshan Tang, Elliot Chaikof, Xue-Long Sun

2. Protecting-group-free Synthesis of Well-defined Glycopolymers Featuring Negatively Charged Oligosaccharides

Luca Albertin

3. Glycopolymers Prepared by Ring-opening Metathesis Polymerization Followed by Glycoconjugation Using a Triazole Forming 'Click' Reaction

Ronald Okoth and Amit Basu

4. Protecting-group-free Synthesis of Glycopolymers and Their Binding Assay with Lectin and Influenza Virus

Tomonari Tanaka, Tadanobu Takahashi, and Takashi Suzuki

5. Carbohydrate-based Initiators for the Cationic Ring-opening Polymerization of 2-Ethyl-2-oxazoline

Christine Weber,Michael Gottschaldt, Richard Hoogenboom, Ulrich S. Schubert

6. Heterofunctional Glycopolypeptides by Combination of Thiol-ene Chemistry and NCA Polymerization

Kai-Steffen Krannig and Helmut Schlaad

7. Preparation of Proteoglycan Mimetic Graft Copolymers

Matt J. Kipper and Laura W. Place

Part II: Glycopolymer Nanoparticle Conjugates

8. Galactosylated Polymer Nano-objects by Polymerization-Induced Self-Assembly, Potential Drug Nanocarriers

Mona Semsarilar, Irene Canton, and Vincent Ladmiral

9. Synthetic Approach to Biotinylated Glyco-Functionalized Quantum Dots: A New Fluorescent Probes for Biomedical Applications

Christian K. Adokoh, James Darkwa, and Ravin Narain

10. Surface Modification of Polydivinylbenzene Microspheres with a Fluorinated Glycopolymer using Thiol-Halogen Click Chemistry

Wentao Song and Anthony M. Granville

11. Glycopolymer-grafted Polymer Particles for Lectin Recognition

Michinari Kohri, Tatsuo Taniguchi, and Keiki Kishikawa

12. Synthesis of Non-spherical Glycopolymer-decorated Nanoparticles: Combing Thiol-ene with Catecholic Chemistry

Xiao Li, Weidong Zhang,and Gaojian Chen

13. Synthetic Approach To Glycopolymer Base Nanoparticle Gold(I) Conjugate: A New Generation Of Therapeutic Agents

Christian K. Adokoh, James Darkwa, and Ravin Narain

14. Multivalent Glycopolymer-coated Gold Nanoparticles

Sarah-Jane Richards, Caroline I. Biggs, and Matthew I. Gibson

Part III: Surface Immobilized Glycopolymers

15. Modulation of Multivalent Protein Binding on Surfaces by Glycopolymer Brush Chemistry

Kai Yu, A. Louise Creagh, Charles A. Haynes, Jayachandran N. Kizhakkedathu

16. Oriented Immobilized Sialyloligo-Macroligand Microarray

Satya Nandana Narla and Xue-Long Sun

17. Glycocalyx Remodeling with Glycopolymer-based Proteoglycan Mimetics

Mia L. Huang, Raymond A. A. Smith, Greg W. Trieger, and Kamil Godula