Main description:

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-

Contents:

Antibodies; Matthew R. Kaser and Gary C. Howard

Antigens; Paul Algate, Jory Baldridge, and Sally Mossman

Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman

Production of Polyclonal Antibodies; Lon V. Kendall

Production of Monoclonal Antibodies; Kathleen C. F. Sheehan

Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith

Purification and Characterization of Antibodies; Joseph P.
Chandler

Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu

Chemical and Proteolytic Modification of Antibodies; George P. Smith

Applications; Lee Bendickson and Marit Nilsen-Hamilton

Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele

Immunoelectron Microscopy; Sara E. Miller and David N. Howell

Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze

ELISAs; John Chen and Gary C. Howard

Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard