Main description:

The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.

Contents:

1. Breast Cancer Stem Cells: Current Advances and Clinical Implications
Ming Luo, Shawn G. Clouthier, Yadwinder Deol, Suling Liu, Sunitha Nagrath, Ebrahim Azizi, and Max S. Wicha

2. A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture
Naoko Kogata and Beatrice A. Howard

3. FACS Sorting Mammary Stem Cells
Oihana Iriondo, Miriam Rabano, and Maria del Mar Vivanco

4. Side Population
Fariba Behbod and Maria del Mar Vivanco

5. Single Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing
Ana M. Aransay, Laura Barcena, Aintzane Gonzalez-Lahera, and Nuria Macias-Camara

6. Shotgun Proteomics on Tissue Specimens Extracted with Acid Guanidinium-Thiocyanate-Phenol-Chloroform
Rene B. H. Braakman, Anieta M. Sieuwerts, and Arzu Umar

7. Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments
Tommaso De Marchi, Eric Kuhn, Steven A. Carr, and Arzu Umar

8. Lentiviral Transduction of Mammary Epithelial Cells
Richard Iggo and Elodie Richard

9. The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads
Marisa M. Faraldo, Marina A. Glukhova, and Marie-Ange Deugnier

10. Humanization of the Mouse Mammary Gland
Wronski A., Arendt L.M., and Kuperwasser C.

11. Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles
Renee van Amerongen

12. Modeling the Breast Cancer Bone Metastatic Niche in Complex Three Dimensional Co-Cultures
Rebecca Marlow, Gabriela Dontu

13. Mammary Cancer Stem Cells Re-Initiation Assessment at the Metastatic Niche: The Lung and Bone
Marc Guiu, Enrique J. Arenas, Sylwia Gawrzak, Milica Pavlovic, and Roger R. Gomis

14. Nanomechanical Characterization of Living Mammary Tissues by Atomic Force Microscopy
Marija Plodinec and Roderick Y. H. Lim

15. Mathematical Modeling as a Tool to Understand Cell Self-Renewal and DifferentiationPhilipp Getto and Anna Marciniak-Czochra

16. Mammary Stem Cells: A Clinicians View
Jose Schneider