Main description:

This second edition is thoroughly revised, updated and enlarged, for the readers. In this second edition, six new chapters have been added: Bone marrow trephine biopsy; Tru-cut biopsy or Core needle biopsy; Diagnostic use of immunhistochemistry in surgical pathology; Microarrays and DNA sequencing; Microdissection and different types of polymerase chain reaction; and Quality control in histopathology.

The chapter on Frozen Section has been fully revised and more practical information have been included, keeping in mind the problems faced by the users during cryostat/frozen sections. Molecular diagnostic changes at a glance in different diseases, tumors and hereditary diseases have been added in the Appendix in Tabular form (almost like a new chapter!) and hope it will be useful for different examinations as last minute revision.

Most of these new chapters have been added in this second edition at the request of readers, especially postgraduate students who are pursuing an MD in Pathology.

Contents:

1. Tissue Fixation and Fixatives

2. Tissue Processing, Microtomy and Paraffin Sections

3. Cryostat and Frozen Section

4. Staining of Tissues-Basic Concept

5. Routine Hematoxylin and Eosin Stain

6. Carbohydrates

7. Lipids

8. Proteins and Nucleic Acid Staining

9. Connective Tissue Staining

10. Pigments and Minerals

11. Bone and Decalcification

12. Nervous System

13. Some Special Stains

14. Enzyme Histochemistry and Histochemical Stains

15. Immunohistochemistry

16. Cell Block and Diagnostic Cytopathology

17. Plastic/Resin Embedding Media

18. Electron Microscopy

19. Immunofluorescence Techniques

20. Museum Technique

21. Light Microscopy

22. Molecular Diagnostic Techniques and Its Applications

23. In Situ Hybridization and Fluorescent In Situ Hybridization

24. Bone Marrow Trephine Biopsy

25. Tru-cut Biopsy or Core Needle Biopsy

26. Diagnostic Use of Immunohistochemistry in Surgical Pathology

27. Microarrays and DNA Sequencing

28. Microdissection and Different Types of Polymerase Chain Reaction

29. Quality Control in Histopathology

Appendices

Glossary

Index