Main description:

Marine enzymes and specialized metabolism - Part B, Volume 605 in the Methods in Enzymology series, highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. Unique sections in this new release include discussions on polysaccharide-degrading enzymes from marine gastropods, radical SAM epimerases from sponge microbes, DMS/P demethylase in bacteria, reconstitution of particulate methane monooxygenase into membrane mimetics, the structure and function of cyanobactin enzymes, marine cyanobacterial polyketide beta-branching enzymology, marine cyanobacterial PKS-NRPS enzymology and structural biology, biochemical profiling of DMSP lyases, and more.

Contents:

1. Polysaccharide-degrading enzymes from marine gastropods Takao Ojima 2. Radical SAM epimerases from sponge microbes Joern Piel 3. DMS/P demethylase in bacteria Chris Reisch 4. Reconstitution of particulate methane monooxygenase into membrane mimetics Amy C. Rosenzweig 5. Structure and function of cyanobactin enzymes Eric W. Schmidt 6. Marine cyanobacterial polyketide beta-branching enzymology David Sherman 7. Marine cyanobacterial PKS-NRPS enzymology and structural biology Janet Smith 8. Biochemical profiling of DMSP lyases Dan Tawfik 9. Preparation and characterization of the enterocin favorskiiase flavoprotein EncM and its distinctive flavin-N5-oxide cofactor Robin Teufel 10. Unusual "head-to-torso"-coupling of terpene precursors as a new strategy for the structural diversification of natural products Robin Teufel 11. Soluble proton ATPases from marine organisms Martin Tresguerres 12. Prochlorosin enzymology in marine cyanobacteria Wilfred van der Donk 13. The Biosynthetic Insights on MIsakinolide and Calyculin in Candidatus 'Entotheonella' Toshiyuki Wakimoto 14. Algal vanadium-dependent haloperoxidases Ron Wever 15. Methane biosynthesis and phosphonates in pelagic bacteria David Zechel 16. Terminal alkyne biosynthesis in marine microbes Wenjun Zhang