Main description:

This volume presents new developments in the field of mass spectrometry imaging, covering imaging, software, data analysis, new instrumentations, and new methodological approaches. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.

Contents:

1. Current Status and Future Prospects of Mass Spectrometry Imaging of Small Molecules

Victoria L. Brown and Lin He

2. Sample Preparation for 3D SIMS Chemical Imaging of Cells

Nicholas Winograd and Anna Bloom

3. TOF-SIMS Imaging of Lipids on Rat Brain Sections

David Touboul and Alain Brunelle

4. MALDI-MS Assisted Molecular Imaging of Metabolites in Legume Plants

Erin Gemperline and Lingjun Li

5. MALDI Mass Spectrometry Imaging of Lipids and Primary Metabolites on Rat Brain Sections

David Touboul and Alain Brunelle

6. Multiplex MALDI-MS Imaging of Plant Metabolites Using a Hybrid MS System

Andrew R. Korte, Gargey B. Yagnik, Adam D. Feenstra, and Young Jin Lee

7. DESI Imaging of Small Molecules in Biological Tissues

Elaine C. Cabral and Demian R. Ifa

8. Desorption Electrospray Ionization Imaging of Small Organics on Mineral Surfaces

Rachel V. Bennett and Facundo M. Fernandez

9. Imaging of Plant Materials Using Indirect Desorption Electrospray Ionization Mass Spectrometry

Christian Janfelt

10. Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Ingela Lanekoff and Julia Laskin

11. Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for Molecular Imaging of Small Molecules on Tissues

Min-Zong Huang, Siou-Sian

Jhang, and Jentaie Shiea

12. Automated Cell-By-Cell Tissue Imaging and Single Cell Analysis for Targeted Morphologies by Laser Ablation Electrospray Ionization Mass Spectrometry

Hang Li, Brian K. Smith, Bindesh Shrestha, Laszlo Mark, and Akos Vertes

13. Laser Ablation Sample Transfer for Mass Spectrometry Imaging

Sung-Gun Park and Kermit K. Murray

14. Nanostructure-Based Mass Spectrometry: High Sensitivity, Enzyme Activity Analysis and Metabolite Imaging

Michael E. Kurczy, Trent R. Northen, Sunia A. Trauger, and Gary Siuzdak

15. Nanostructure-Initiator Mass Spectrometry (NIMS) for Molecular Mapping of Animal Tissues

Tara N. Moening, Victoria L. Brown, and Lin He

16. Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging

Michihiko Waki, Eiji Sugiyama, Takeshi Kondo, Keigo Sano, and Mitsutoshi Setou

17. Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules

Victoria L. Brown, Qiang Liu, and Lin He

18. Laser Desorption Postionization Mass Spectrometry Imaging of Biological Targets

Artem Akhmetov, Chhavi Bhardwaj, and Luke Hanley

19. Data Processing and Analysis for Mass Spectrometry Imaging

Jiangjian Liu, Xingchuang Xiong, and Zheng Ouyang.