Main description:

This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.

Contents:

1. Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae

Yona Kassir and David T. Stuart

2. Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe

Randy W. Hyppa, Kyle R. Fowler, and Gerald R. Smith

3. Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast

Isabel Lam, Neeman Mohibullah, and Scott Keeney

4. Ribosome Profiling for the Analysis of Translation During Yeast Meiosis

Caia Duncan and Juan Mata

5. Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation

David T. Stuart

6. Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora

Frederick J. Bowring, P. Jane Yeadon, and David E.A. Catcheside

7. High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae

Yona Kassir

8. Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast

Amy J. MacQueen and Beth Rockmill

9. In Vivo Imaging of Budding Yeast Meiosis

Michael Pollard and Jennifer C. Fung

10. Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I

Lorinda K. Anderson

11. Flow Cytometry for the Isolation and Characterization of Rodent Meiocytes

Adriana Geisinger and Rosana Rodriguez-Casuriaga

12. Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH

Harry Scherthan 13. Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy

Simon I.R. Lane, Stephen Crouch, and Keith T. Jones

14. Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes

Stacie E. Hughes and R. Scott Hawley

15. Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes

Matthew S. Savoian

16. Drosophila Male Meiosis

Silvia Bonaccorsi and Maurizio Gatti

17. Analysis of Chromatin Dynamics During Drosophila Spermatogenesis

Tim Hundertmark, Ina Theofel, Zeynep Eren-Ghiani, David Miller, and Christina Rathke

18. Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model< Martin A. White, Shunxin Wang, Liangran Zhang, and Nancy Kleckner

19. A Computational Approach to Study Gene Expression Networks

Amir Rubinstein and Yona Kassir