Main description:

Studies of membrane transporters have had a great impact on our understanding of human diseases and the design of effective drugs. In Membrane Transporters in Drug Discovery and Development: Methods and Protocols, expert researchers provide practical methodologies of the ongoing research on membrane transporters, considering applications of transporter technologies in drug discovery and development. Chapters include new and useful fields and methodologies, including pharmacogenomics, nutrigenomics, systems biology, bioinformatics, nuclear magnetic resonance (NMR), imaging, and quantitative real-time-PCR. Transporter studies in drug discovery and development for various diseases are discussed, including neuropsychiatric disorders, cardiovascular diseases, ophthalmic diseases, cancer, and diabetes. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and current, Membrane Transporters in Drug Discovery and Development: Methods and Protocols delivers a collection of practical protocols that can be used immediately in the lab, along with critical surveys of key topics by leading researchers in the field.

Contents:

Membrane Transporters in Drug Discovery and Development: Methods and Protocols

Table of Contents:

Table of Contents
Preface
Contributors

1. Membrane Transporters and Drug Development: Relevance to Pharmacogenomics, Nutrigenomics, Epigenetics, and Systems Biology
Qing Yan

2. Bioinformatics for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML
Qing Yan

3. Multi Drug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers that Include Drug Exporters
Ming Ren Yen, Jonathan Chen, Jose L. Marquez, Eric I. Sun and Milton H. Saier, Jr.

4. Targeting Drug Transporters - Combining in Silico and In Vitro Approaches to Predict in Vivo
Praveen M. Bahadduri, James E. Polli, Peter W. Swaan and Sean Ekins

5. Methods to Evaluate Transporter Activity in Cancer
Keisuke Mitsuoka, Douglas D. Ross, and Takeo Nakanishi

6. Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR
Anna Maria Calcagno and Suresh V. Ambudkar

7. Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters
Frances J. Sharom, Ronghua Liu and Balpreet Vinepal

8. A Model for Transport Studies of the Blood-Brain Barrier
Dennis J. Bobilya

9. Genetic Variants in the Vesicular Monoamine Transporter 1 (VMAT1/SLC18A1) and Neuropsychiatric Disorders
Falk W. Lohoff

10. Equilibrium Binding and Transport by Vesicular Acetylcholine Transporter
Parul Khare, Aubrey R. White, Anuprao Mulakaluri, and Stanley M. Parsons

11. ABC Transporters in Ophthalmic Disease
Corey Westerfeld

12. Imaging of Protein Translocation insitu in Skeletal Muscle of Living Mice
Hans P.M.M Lauritzen

13. Glucose Transporters in Parasitic Protozoa
Scott M. Landfear

14. NMR Studies of Membrane Proteins
Gabriel A. Cook and Stanley J. Opella

15. Site-Directed Mutagenesis in the Study of Membrane Transporters
Audra A. McKinzie, Renae M. Ryan, and Robert J. Vandenberg

16. Xenopus laevis Oocytes
Stefan Broeer

17. Measurement of Intracellular pH
Frederick B. Loiselle and Joseph R. Casey

18. Measurement of Plasma Membrane Calcium-Calmodulin Dependent ATPase (PMCA) Activity
Tamer M. A. Mohamed, Florence M. Baudoin-Stanley, Riham Abou-Leisa, Elizabeth Cartwright, Ludwig Neyses, and Delvac Oceandy

19. Assessment of the Contribution of the Plasma Membrane Calcium ATPase, PMCA, Calcium Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging
Chris J. Roome and Ruth M. Empson