Main description:

This volume explores methods used to examine metal levels and distribution in brain tissue or brain-derived cells. The chapters in this book discuss the use of fluorescent metal probes, synchrotron-based X-ray microscopy, ICP-MS, laser ablation-ICP-MS, laser-based tissue microdissection, MRI image analysis, fractionation of cell tissue samples for metal analysis, and metal treatment of cells. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Practical and cutting-edge, Metals in the Brain: Measurement and Imaging is a valuable resource for researchers in the rapidly growing area of neuroscience research.

Contents:

Neuromethods Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. Introduction and Overview
Anthony R. White

2. X-Ray Microscopy for Detection of Metals in the Brain
Joanna F. Collingwood and Freddy Adams

3. Imaging Metals in the Brain by Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry
Dominic J. Hare, Bence Paul, And Philip A. Doble

4. Fluorescent Probes for the Analysis of Labile Metals in the Brain Cells
Jacek L. Kolanowski, Clara Shen, and Elizabeth J. New

5. Probing Biological Trace Metals with Fluorescent Indicators
Christoph J. Fahrni, Daisy Bourassa, and Ryan Dikdan

6. Microdissection of Brain Tissue to Resolve the Comparative Dissemination of Metal Concentrations in Neurodegenerative Diseases, Focusing on Manganese
Marcus W. Brazier

7. Analysis of Trace Elements and Metalloproteins in Fractionated Human Brain Samples using Size Exclusion Inductively Couples Mass Spectrometry
Adam Gunn and Blaine R. Roberts

8. Size-Fractionation of Metal Species from Serum Samples for Studying Element Biodistribution in Alzheimer's Disease
Raul Gonzalez-Dominguez

9. Lead Uptake and Localization in Glial Cell Cultures
Rola Barhoumi, Robert Taylor, and Evelyn Tiffany-Castiglioni

10. Evaluating Iron Flux in the Brain
Bruce X. Wong, Linh Q. Lam, Andrew Tsatsanis, and James A. Duce

11. Current Methods used to Probe and Quantify Intracellular Total and Free Zn(II) Dynamics, and Subcellular Distribution in Cultured Neurons
Yan Qin, Kyle R. Gee, Qiaoling Jin, Barry Lai, Cheng Qian, and Robert A. Colvin

12. Monitoring Intracellular Zn2+ using Fluorescent Sensors: Facts and Artifacts
Lech Kiedrowski

13. Measuring Changes in Brain Manganese or Iron using Magnetic Resonance Imaging (MRI)
Kimberly L. Desmond and Nicholas A. Bock

14. Treatment and Measurement of Metals in Brain Cell Cultures
Xin Yi Choo and Alexandra Grubman