Main description:

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Contents:

1. In Vitro Invasion Assay Using MatrigelTM - a Reconstituted Basement Membrane Preparation

Debbie M S Hall and Susan A Brooks

2. Single Cell and Spheroid Collagen Type I Invasion Assay

Olivier De Wever , An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems, Marc Bracke

3. Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells

Deepashree Bapu, Munira Khadim and Susan A. Brooks

4. Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow Conditions

Ulrich Richter and Udo Schumacher

5. Adhesion of Tumour Cells to Matrices and Endothelium

Clara M Yates, Helen M McGettrick, Gerard B Nash, and G. Ed Rainger

6. Cell Aggregation Assays

Delphine Debruyne, Tom Boterberg, and Marc E. Bracke

7. Chick Heart Invasion Assay

Marc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, and Marc M. Mareel

8. Computer Simulation of the Metastatic ProgressionGero Wedemann, Anja Bethge, Volker Haustein, and Udo Schumacher

9. Theoretical Considerations In Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice

Sue A. Watson and Rajendra Kumari

10. Syngeneic Murine Metastasis Models: B16 MelanomaRaffaella Giavazzi and Alessandra Decio

11. Imageable Clinically-relevant Mouse Models of Metastasis

Robert M. Hoffman

12. Imaging Metastatic Cell Trafficking at the Cellular Level in vivo with Fluorescent Proteins

Robert M. Hoffman

13. Ultrasound Techniques for the Detection of Tumours and Metastases in Small Animals

Michael Didie and Wolfram-Hubertus Zimmermann

14. The PFP/RAG2 Double Knock Out Mouse in Metastasis Research: Small Cell Lung Cancer and Prostate Cancer

Imke Muller and Sebastian Ullrich

15. Ultrasound Guided Intracardial Injection and In-vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Haematogenous Metastases

Johannes Salamon and Kersten Peldschus

16. Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer

Kersten Peldschus and Harald Ittrich

17. Spontaneous and Experimental Metastasis Models: Nude Mice

Janet E Price

18. Identifying the Origin and Phenotype of Cells in Tumour Xenografts

Rosemary Jeffery, Pooja Seedhar, and Richard Poulsom