Main description:

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Contents:

1. Basic Immunocytochemistry for Light Microscopy

Susan A. Brooks

2. Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues

Susan A. Brooks & Debbie M.S. Hall

3. Histopathological Assessment of Metastasis

Derek E Roskell and Ian D Buley

4. Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells

Stephan Braun and Klaus Pantel

5. The Polymerase Chain Reaction (PCR)

Hazel M. Welch

6. SDS-PAGE and Western Blotting Techniques

Christine Blancher and Rob Mc Cormick

7. 2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins

Miriam V Dwek and Diluka Peiris

8. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography

Marta Toth, Anjum Sohail, and Rafael Fridman

9. Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer

Michael R. Dashwood and Marilena Loizidou

10. Fluorescence in situ Hybridization (FISH) for Cancer Related Studies

Lyndal Kearney and Janet Shipley

11. Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells

Julien Saint Guirons and Bjoern Ingemarsson

12. Cell Separations by Flow Cytometry

Derek Davies

13. Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cells Cultures

Matthias Christgen, Matthias Ballmaier, Ulrich Lehmann and Hans Kreipe

14. Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours

Caroline Schreiber, Kirsten Vormbrockand Ulrike Ziebold

15. High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing (TM)

Ulrich Lehmann, Cord Albat, Hans Kreipe

16. RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example

Daniel Wicklein

17. Galectin-3-Binding and Metastasis

Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz

18. Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation

Simon Fry, Babak Afrough, Anthony Leathem and Miriam V Dwek