Main description:

Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.

Contents:

1. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections

Elizabeth Palavecino

2. Community-Associated Methicillin Resistant Staphylococcus aureus Case Studies

Madeleine G. Sowash and Anne-Catrin Uhlemann

3. Rapid Methods for Detection of MRSA in Clinical Specimens

Elizabeth Palavecino

4. Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA)

Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson

5. Internal Transcribed Spacer (ITS)-PCR Identification of MRSA

Shin-ichi Fujita

6. Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates

Yiping He, Yanping Xie, and Sue Reed

7. Multilocus Sequence Typing (MLST) of Staphylococcus aureus

Nicholas A. Saunders and Anne Holmes

8. Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA

Teruyo Ito, Kyoko Kuwahara, Yuki Katayama, Yuki Uehara, Xiao Han, Yoko Kondo, and Keiichi Hiramatsu

9. Genetic Interruption of Target Genes for Investigation of Virulence Factors

Adhar C. Manna

10. Molecular Analysis of Staphylococcal Superantigens

Wilmara Salgado-Pabon, Laura C. Case-Cook, and Patrick M. Schlievert

11. Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells

Junshu Yang and Yinduo Ji

12. Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus

James E. Cassat, Mark S. Smeltzer, and Chia Y. Lee

13. Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies

Ting Lei, Aaron Becker, and Yinduo Ji

14. Proteomic Approach to Investigate Pathogenicity and Metabolism of Methicillin-Resistant Staphylococcus aureus

Patrice Francois, Alexander Scherl, Denis Hochstrasser, and Jacques Schrenzel

15. Metobolomic Investigation of Methicillin Resistant Staphylococcus aureus

Ting Lei, Lei Wang, Chi Chen, and Yinduo Ji

16. Treatment of Infections Due to Resistant Staphylococcus aureus

Gregory M. Anstead, Jose Cadena, and Heta Javeri

17. Anti-Infective Drug Development for MRSA

Anu Daniel

18. Animal Models in Drug Development for MRSA

Andrea Marra