Main description:

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Contents:

Neuromethods Series Preface...
Preface...
Table of Contents...
Contributing Authors...1. Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord
Irit Reichenstein and Eran Hornstein

2. Protocol for High Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons
Tal Yardeni and Eran Hornstein

3. Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-Radioactive Fluorescence Method
Anna Emde, Natalia Rivkin, Mark A. Behlke, and Eran Hornstein

4. Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Protocol
Danielle Twaroski, Yasheng Yan, Jessica M. Olsen, Mingyu Liang, Zeljko J. Bosnjak, and Xiaowen Bai

5. MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation
Lara Stevanato, Caroline Hicks, Lavaniya Thanabalasundaram, and John D. Sinden

6. Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation
Patricia Garcez, Francois Guillemot, and Federico Dajas-Bailador

7. Functional Analysis of Cortical Neuron Migration Using miRNA Silencing
Pierre-Paul Prevot, Marie-Laure Volvert, Alexander Deiters, and Laurent Nguyen

8. Continuous Delivery of Oligonucleotides into the Brain
Ilya A. Vinnikov, Andrii Domanskyi, and Witold Konopka

9. Isolating and Screening Subcellular miRNAs in Neuron
Min Jeong Kye

10. Experimental Methods for Functional Studies in MicroRNAs in Animal Models of Psychiatric Disorders
Vladimir Jovasevic and Jelena Radulovic

11. Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs
Hak Hee Kim, Monichan Phay, and Soonmoon Yoo

12. In Ovo Electroporation of miRNA Plasmids to Silence genes in a Temporally and Spatially Controlled Manner
Nicole H. Wilson and Esther T. Stoeckli

13. Three Dimensional Cell Culture of Human Neural Stem Cells using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses
Geun-woo Jin, Weili Ma, and Won H. Suh

14. Measuring miRNA Mediated Translational Regulation with Live Cell Imaging
Min Jeong Kye