Main description:

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Contents:

1. Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease

David A. Dunn and Carl A. Pinkert

2. Mitochondrial Transplantation for Ischemia Reperfusion Injury

Ilias P. Doulamis and James D. McCully

3. Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device

Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, and Mizuo Maeda

4. Lipophilic Conjugates for Carrier-Free Delivery of RNA Importable Into Human Mitochondria

Ilya Dovydenko, Mariya Meschaninova, Anne-Marie Heckel, Ivan Tarassov, Alya Venyaminova, and Nina Entelis

5. Drug Discovery Assay to Identify Modulators of the Mitochondrial Ca2+ Uniporter

Daniela M. Arduino, Valerie Goh, Dejana Mokranjac, and Fabiana Perocchi

6. Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice

Thomas Kolbe, Ralf Steinborn, and Joerg P. Burgstaller

7. Electron Attachment to Isolated Molecules as a Probe To Understand Mitochondrial Reductive Processes

Stanislav A. Pshenichnyuk and Alberto Modelli

8. Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation

Matthias Hecker, Natascha Sommer, and Konstantin Mayer

9. Assessment of the Effects of Drugs on Mitochondrial Respiration

Jana Hroudova and Zdenek Fisar

10. The Ndufs4 Knockout Mouse: A Dual Threat Model of Childhood Mitochondrial Disease and Normative Aging

Anthony S. Grillo, Alessandro Bitto, and Matt Kaeberlein

11. Suborganellar Localisation of Mitochondrial Proteins and Transcripts in Human Cells Anna Smirnova, Ludovic Richert, Alexandre Smirnov, Yves Mely, and Ivan Tarassov

12. In vivo Assessment of Mitochondrial Oxygen Consumption

Floor A. Harms and Egbert G. Mik

13. Update of Mitochondrial Network Analysis by Imaging: Proof of Technique in Schizophrenia

Yekaterina Yatchenko and Dorit Ben-Shachar

14. Heterologous Inferential Analysis (HIA) and other Emerging Concepts

In Understanding Mitochondrial Variation in Pathogenesis: There is no More Low Hanging Fruit

Anton Vila-Sanjurjo, Paul M. Smith, and Joanna L. Elson

15. Accurate Measurement of Cellular and Cell-Free Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR

Hannah Rosa and Afshan Malik

16. Measurement of Mitochondrial Respiration in Platelets

Zdenek Fisar and Jana Hroudova

17. Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria Yaschar Kabiri, Carola Eberhagen, Sabine Schmitt, Percy A. Knolle, and Hans Zischka

18. Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells

Madhavee Thumiah-Mootoo, Tina Podinic, and Mireille Khacho

19. Single Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease

Laura J. Bailey, Joanna L. Elson, and Ilse S. Pienaar

20. A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology

Sophie Dhorne-Pollet, Nicolas Pollet, and Eric Barrey

21. Mitochondrial DNA as a Sensitive Biomarker of UV-induced Cellular Damage in Human Skin

Amy Bowman and Mark A. Birch-Machin

22. Isolation of Mitochondria-associated ER Membranes (MAMs), Synaptic MAMs and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells

Ida Annunziata, Jason Andrew Weesner, and Alessandra d'Azzo

23. Mitochondrial Diagnostics: A Discovery-Based Biochemical Platform for Phenotyping Human Peripheral Blood Cell Mitochondria

Margaret A. M. Nelson and Kelsey H. Fisher-Wellman

24. Bioenergetic Profiling Of Human Pluripotent Stem Cells

Gizem Inak-Girrbach, Marie-Therese Henke, and Alessandro Prigione

25. Integrative Methods for Studying Cardiac Energetics

Philippe Diolez, Veronique Deschodt-Arsac, Guillaume Calmettes, Gilles Gouspillou, Laurent Arsac, Pierre Jais, Michel Haissaguerre, and Pierre dos Santos

26. Isolation of Mitochondria from Retinal Pigment Epithelial Cell Cultures and an Application Of High Resolution Respirometric Assay (XFe96 Seahorse assay)

Masaaki Ishii, Gyda Beeson, Craig Beeson, and Barbel Rohrer

27. The Isolation and Deep Sequencing of Mitochondrial DNA

Alexander G. Bury, Fiona M. Robertson, Angela Pyle, Brendan A. I. Payne, and Gavin Hudson

28. Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens

Gabriel Arismendi-Morillo