Main description:

Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism. In, Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition gathers a wide array of strategies and protocols for identifying transposable elements (TEs) and their evolutionary derivatives, and for studying genome structure, function, and evolution. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Through and intuitive Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition aids scientists in the continuing study of in silico tools, strategies and protocols that are complementary of those described in the first volume.

Contents:

1. Transposable Elements as Tooks for Reshaping the Genome: it's a Huge World After All! Solenne Bire and Florence Rouleux-Bonnin

2. Using and Understand RepeatMasker Sebastien Tempel

3. Roadmap for Annotating Transposable Elements in Eukaraote Genomes Emmanuelle Permal, Timothee Flutre, Hadi Quesneville

4. To Detect and to Analyzy Sequence Repeates Whatever their Origin Jacques Nicolas

5. Exploring Bacterial Insertion Sequences with ISfinder: Objective Developments P. Siguier, A. Varani, J. Perochon and M. Chandler

6. Methods and Softward in NGS for TE Analysis Cristian Chaparro and Francois Sabot

7. The Application of LTR Retrotransponsons As Molecular Markers in Plants Alan H. Schulman, Andrew J. Flavell, Etienne Paux and T. H. Noel Ellis

8. Individual Analysis of Transposon Polymorphisms by AFLP Ssusanta K. Behura

9. Construction of a Library of Random Mutants in the Spirochete Leptospira Biflexa using a Mariner Transposon Leyla Slamti and Mathieu Picardeau

10. Ac-Ds Solutions for Rice Insertion Mutagenesis Emmanuel Guiderdoni and Pascal Gantet

11. Engineering the Caenorhabditis elegans Genome by Mos1 Induced Transgene-Instructed Gene Converstion (MosTIC) Valerie JP Robert

12. Genome-wide Manipulations of Drosophila Melanogaster with Transposons, Flp Recombinases and FC31 Integrase Koen J.T. Venken and Hugo J. Bellen

13. The Sleeping Beauty Transposon Toolbox Ismahen Ammar, Zsuzanna Izsvak and Zoltan Ivics

14. Piggybac Took Box Mario Di Matteo, Janka Matrai, Tewodros Firdissa, Thierry VandenDriessche, Marinee K.L. Chuah

15. Insertion Site Patter: Global Approach by Linear Amplification-mediated PCR (LAM-PCR) and Mass Sequencing Cynthia C. Bartholomae, Hanno Glimm, Christof von Kalle, Manfred Schmidt

16. Comprehensive DNA Methyalation Profiling of Human Repetitive DNA Elements using a MeDIP-on-RepArray Assay Eric Gilson and Beatrice Horard

17. Novel Approach for the Development of New Antibodies Directed Against Transposase-derived Proteins Encoded by human Neogenes

Ahmend Arnaoty, Bruno Pitard, Benoit Bateau, Yves Bigot and Thierry Lecomte