Main description:

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors' own research programs.

Contents:

Molecular Biomethods Handbook 2nd Ed

Contents

PART A: NUCLEIC ACID BIOMETHODS

1 The manipulation of nucleic acids: basic tools and techniques - Gayle Corkill and
Ralph Rapley

2 Restriction Enzymes - Gareth J.S. Jenkins

3 Principles and Medical Applications of the Polymerase Chain Reaction - Birmal D.M.
Theophilus

4 Probe Design, Production, and Applications - Marilena Aquino de Muro

5 Southern Blotting as a Diagnostic Method - Bronwen Harvey and Pirkko Soundy

6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega

7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid
analysis - Kim Hung Leung and Shea Ping Yip

8 Denaturing Gradient Gel Electrophoresis (DGGE) - Jeroen H. Roelfsema and Dorien
J.M. Peters

9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and
Shea Ping Yip

10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic
characterization of different organisms - Lucia Maria da Cunha Galvao and Eliane
Lages-Silva

11 Quantification of mRNA using Real Time RT-PCR - the SYBR solution - David
Sugden and Patricia de Winter

12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing - Anu
Suomalainen and Ann-Christine Syvanen

13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila
Kousoulidou and Philippos C. Patsalis

14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley

15 Comparative Genomic Hybridization in Clinical and Medical Research - Peng-Hui
Wang, Yann-Jang Chen and Chi-Hung Lin

16Subtractive Hybridization - Craig Winstanley

17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire

18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -
Jonathan D. Smith

19 cDNA Microarrays - Phillip G. Febbo

20 Mapping Techniques - Simon G. Gregory

21 Single Nucleotide Polymorphisms Overview - Nameeta Shah

22 Bioinformatics Tools for Gene and Protein Sequence Analysis- Bernd H.A. Rehm and
Frank Reinecke

PART B: PROTEIN AND CELL METHODS

23. Protein Electrophoresis - David Sheehan and Siobhan O'Sullivan
24. Protein blotting - Patricia Gravel
25. Capillary electrophoresis of proteins - Mark Strege
26. Autoradiography and fluorography - Bronwen Harvey
27. Mass spectrometry of proteins & peptides - Kenneth G. Standing
28. Post-translational modifications - Christoph Kannicht and Birte Fuchs
29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung
30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu
31. Glycoprotein analysis - Terry Butters and David C.A. Neville
32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields
33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar
34. Antibody Phage display - Rob Aitken
35. Protein engineering - Thomas Willemsen, Urs B. Hagemann,
Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Muller and Katja M. Arndt
36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Muller
37. Enzyme Linked Immunosorbent Assay - John Crowther
38. Epitope mapping - Glenn E. Morris
39.