Main description:

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

Contents:

1. Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration

Irene Castellano-Pellicena and M. Julie Thornton

2. Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

Rachael Williams and M. Julie Thornton

3. Isolation of Epidermal and Hair Follicle Melanocytes

Richard Baker and M. Julie Thornton

4. Isolation and Culture of Human Skin Mast Cells

Hanna Siiskonen and Joerg Scheffel

5. Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines

Gijs Rikken, Hanna Niehues, and Ellen H. van den Bogaard

6. Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts

Isabelle Petit, Ayelet Levy, and Daniel Aberdam

7. A Method to Investigate the Epidermal Permeability Barrier In Vitro

Yoshikazu Uchida and Anna Celli

8. Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion

Summik Limbu and Claire A. Higgins

9. Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture

Janin Lehmann, Jennifer Gherardini, and Marta Bertolini

10. Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells

Eddy H.C. Wang and Kevin J. McElwee

11. Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata

Markus Fehrholz and Marta Bertolini

12. Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo

Ilaria Piccini and Marta Bertolini

13. Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells

Ekaterina P. Kalabusheva and Ekaterina A. Vorotelyak

14. Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes

Francisco Jimenez

15. Identification of Long Non-Coding RNA by In Situ Hybridization Approaches

Mara Mancini, Anna Maria Lena, and Eleonora Candi

16. Detection of MicroRNAs by In Situ Hybridization in Skin

Maximilian E. Pickup and Mohammed I. Ahmed

17. Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells

Carmit Bar, V. Julian Valdes, and Elena Ezhkova

18. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ

Andrei N. Mardaryev and Michael Y. Fessing

19. Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin

Andrei N. Mardaryev

20. Investigation of Skin Wound Healing Using a Mouse Model

Jianmin Wu and Ning Xu Landen

21. Human Wound Healing Ex Vivo Model with Focus on Molecular Markers

Jennifer Gherardini, Max van Lessen, Ilaria Piccini, Janin Lehmann, and Marta Bertolini

22. Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light

Charles Mignon and Natallia E. Uzunbajakava