Main description:

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.

Contents:

1. Applications of Molecular Genetics to the Study of Asthma

Catalina S. Sanz Lozano, Virginia Garcia-Solaesa, Ignacio Davila Gonzalez, and Maria Isidoro-Garcia

2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach

Belen Garcia Berrocal, Asuncion Garcia-Sanchez, and Maria Isidoro-Garcia

3. Introduction to the Gene Expression Analysis

Ignacio San Segundo-Val and Catalina S. Sanz Lozano

4. Real Time PCR for Gene Expression Quantification in Asthma

Ignacio San Segundo-Val, Virginia Garcia-Solaesa, and Asuncion Garcia-Sanchez

5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma

Ignacio San Segundo-Val and Catalina S. Sanz Lozano

6. Review of Methods to Study Gene Expression Regulation Applied to Asthma

Asuncion Garcia-Sanchez and Fernando Marques Garcia

7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma

Virginia Garcia-Solaesa and Catalina S. Sanz Lozano

8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma

Virginia Garcia-Solaesa and Sara Ciria Abad

9. Chromatin Immunoprecipitation: Application to the Study of Asthma

Asuncion Garcia-Sanchez and Fernando Marques-Garcia

10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols

Asuncion Garcia-Sanchez and Fernando Marques-Garcia

11. Protocols for Exosome Isolation and RNA Profiling

Fernando Marques-Garcia and Maria Isidoro-Garcia

12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell

Elena Marcos-Vadillo and Asuncion Garcia-Sanchez

13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line

Elena Marcos-Vadillo and Asuncion Garcia-Sanchez

14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line

Elena Marcos-Vadillo and Asuncion Garcia-Sanchez

15. Review of Mouse Models Applied to the Study of Asthma

Fernando Marques-Garcia and Elena Marcos-Vadillo

16. General and Specific Mouse Models for Asthma Research

Fernando Marques-Garcia and Elena Marcos-Vadillo

17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma

Fernando Marques-Garcia and Elena Marcos-Vadillo

18. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma

Almudena Sanchez-Martin, Asuncion Garcia-Sanchez, and Maria Isidoro-Garcia