Main description:

In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.

Contents:

Molecular Imaging: Methods and Protocols
Editor Khalid Shah, PhD

TABLE OF CONTENTS

Table of Contents

Preface

Contributors

Imaging Biochemical Pathways and Molecular Events

1. Bioluminescence resonance energy transfer (BRET) Imaging in Plant Seedlings and Mammalian Cells
Mohammed Soutto, Qiguang Xie, Xiaodong Xu, Yunfei Zhang, and Carl Hirschie Johnson

2. Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and Mice
Gary Luker and Kathryn Luker

3. Hybrid Raman-Fluorescence Microscopy on Single Cells using Quantum Dots
Henk-Jan van Manen and Cees Otto

4. Labeling of Mesenchymal Stem Cells with Bioconjugated Quatum Dots
Bhranti S. Shah and Jeremy J. Mao

5. Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence
Jing Lu and Andrew Tsourkas

Imaging in Pre-clinical settings

6. Imaging Fate of Stem Cells at a Cellular Resolution in the Brains of Mice
Khalid Shah

7. Magnetic Resonance Imaging of Brain Inflamation using Microparticles of Iron Oxide
Martina A. McAteer, Constantin von Zur Muhlen, Daniel C. Anthony, Nicola R. Sibson, Robin P. Choudhury

8. Optical Characterization of Arterial Apoptosis
Maarten F. Corsten and Abdelkader Bennaghmouch

9. Intravital Near-Infrared Fluorescence Molecular Imaging of Atherosclerosis
Farouc Jaffer

10. MR Imaging of Transplanted Stem Cells in Myocardial Infarction
Dara L. Kraitchman, Dorota A.. Kedziorek and Jeff W.M. Bulte

11. In Vivo Imaging of the Dynamics of Different Variants of EGFR in Glioblastomas
Khalid Shah

12. Fluorescence lifetime-based optical molecular imaging
Anand T.N. Kumar

Imaging in Clinical Settings

13. PET maging of avb3 Integrins in Cancer Patients
Ambros J. Beer and Markus Schwaiger

14. Quantitative Approaches to Amyloid Imaging
Victor L. Villemagne, Graeme O'Keefe, Rachel S. Mulligan and Christopher C. Rowe

15. Molecular imaging of myocardial remodeling after infarction
Johan W. H. Verjans, Susanne W. M. van de Borne, Leo Hofstra and Jagat Narula

16. Dual-radionuclide Brain SPECT for the Differential Diagnosis of Parkinsonism
Georges El Fakhri and Jinsong Ouyang