Main description:

The third Symposium of the Foundation for Life Sciences was held in February 1983 at the Newport Inn Conference Centre in Sydney. It was direced towards an understanding of the molecular neuropathology of muscle and nerve under a wide variety of conditions that may be induced by external agents or genetic lesions. The first session on experimental neurology explored the processes involved in maintenance of nerve and muscle function. This included many papers on myelination, studies on immune reactions affecting nerves, on synapses, and on neuronal development. This section was expanded to explore the control of muscle function in nerves, including a discussion on cross reinnervation. Toxic models of disease in the nervous system were then discussed, including pathological states induced by physical agents such as kainic acid, diphtheria toxin, and IDPN. A new dimension was added to the Symposium when for the first time psychologists participated and contributed to the session on external stressors and their effects on behavior. Heavy metals, herbicides, repetitive work, anxiety, and their effects on behavior and health were all represented. The discussion in this session attracted much interest from the participants, particularly the basic scientists.

Contents:

Amino Acid Analysis Book

Chapters In Order

1. An introduction to amino acid analysis - Margaret Tyler

2. Amino acid analysis, using post-column ninhydrin detection, in a biotechnology laboratory - Frank D. Macchi, Felicity J. Shen, Rodney G Keck, and Reed Harris

3. Purification of proteins using UltraMacro spin columns or ProSorb sample preparation cartridges for amino acid analysis - Li Zhang

4. Amino acid analysis using pre-column derivatisation with 6-aminoquinolyl-N-hydroxysuccinimidyl carbonate - Steven A Cohen

5. Amino acid analysis by high-performance liquid chromatography after derivatization with 1-flouro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's Reagent) - Sunil Kochhar, Barbara Mouratou and Philipp Christen

6. The analysis of amino acids using pre-column derivatisation, HPLC and electrochemical detection - C. David Forster and Charles A Marsden

7. Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids - Petr Jandik

8. Application of AQC-amino acid analysis - Ion-pair chromatography for identification of picomolar-order protein on a PVDF membrane - Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno and Kimie Murayama

9. Capillary gas chromatography analysis of protein and non-protein amino acids in biological samples - Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto and Masami Makita

10. Measurement of blood amino acids in ultrafiltrates by HPLC with automated pre-column O-phthaldialdehyde derivatisation - Hua Liu

11. Determination of standard amino acids in foods by reversed-phase high-performance liquid chromatography (RP-HPLC) with new precolumn dericvatives, butylthiocarbamyl (BTC) and benzylthiocarbamyl (BZTC) derivatives compared to the phyenylcarbamyl derivative and ion exchange chromatography - Kang-Lyung Woo

12. Amino acid measurement in body fluids using PITC derivatives - Roy Sherwood

13. Determination of proteins, phosphatidylethanolamine and phosphatidylserine in lipid-rich materials by analysis of phenylthiocarbamyl derivatives - Margareta Stark and Jan Johansson

14. Analysis of O-phosphoamino acids in biological samples by gas chromatography with flame photometric detection - Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai and Masami Makita
15. Determination of sulfur amino acids, glutathione and related aminothiols in biological samples by gas chromatography with flame photometric detection - Hiroyuki Kataoka, Kiyomi Takagi, Hirofumi Tanaka and Masami Makita

16. Capillary electrophoretic determination of 4-hydroxyproline - Qingyi Chu and Michael Zeece

17. Total plasma homocysteine analysis by HPLC with SBD-F pre-column derivatization - Isabella Fermo and Rita Paroni

18. Determination of early glycation products by mass spectrometry and quantification of glycation mediated protein crosslinks by the incorporation of [14C] lysine into proteins - Malladi Probhakaram, Beryl J Ortwerth and Jean B Smith