Main description:

With genetic engineering, systems explored in this book now exist allowing
for the simple, efficient, and near universally precise genetic manipulation
directly in any organism, including the mouse. Herein, these models are applied
to a wide field of disease areas, including diabetes, cardiovascular disease,
skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal
disorders, as well as various behavioral models. Written for the highly
successful Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical
and fully updated, Mouse Models for Drug
Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview
of techniques to utilize the many possibilities of mice in the drug development
process.

Contents:

1. Genetically Defined Strains in Drug
Development and Toxicity Testing

Michael F.W. Festing

2. Simple, Efficient CRISPR-Cas9
Mediated Gene Editing in Mice: Strategies and Methods

Benjamin E. Low, Peter M. Kutny, and
Michael V. Wiles

3. Mouse Sperm Cryopreservation and
Recovery of Genetically Modified Mice

Benjamin E. Low, Rob A. Taft, and
Michael V. Wiles

4. Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment
of Hematopoietic Stem Cells

Suheyla Hasgur, Ken Edwin Aryee,
Leonard D. Shultz, Dale L. Greiner, and Michael A. Brehm

5. Generation of Human Liver Chimeric
Mice for the Study of Human Hepatotropic Pathogens

Markus von Schaewen, Gabriela Hrebikova, and Alexander Ploss

6. Human FcRn Transgenic Mice for
Pharmacokinetic Evaluation of Therapeutic Antibodies

Derry C. Roopenian, Gregory J.
Christianson, Gabriele Proetzel, and Thomas J. Sproule

7. A Humanized Mouse Model to Study
Human Albumin and Albumin Conjugates Pharmacokinetics

Benjamin E. Low and Michael V. Wiles

8. Germ-Free Mice Model for Studying
Host-Microbial Interactions

Yogesh Bhattarai and Purna Kashyap

9. Bridging Mice to Men: Using HLA
Transgenic Mice to Enhance the Future Prediction and Prevention of Autoimmune
Type 1 Diabetes in Humans

David V. Serreze, Marijke Niens,
John Kulik, and Teresa P. DiLorenzo

10. Mouse Models of Type 2 Diabetes
Mellitus in Drug Discovery

Helene Baribault

11. Cholesterol Absorption and
Metabolism

Philip N. Howles

12. Skin Diseases in Laboratory Mice:
Approaches to Drug Target Identification and Efficacy Screening

John P. Sundberg, Kathleen A. Silva,
Lloyd E. King, Jr, and C. Herbert Pratt

13. Chronic Myeloid Leukemia (CML) Mouse
Model in Translational Research

Cong Peng and Shaoguang Li

14. Murine Model for Colitis-Associated
Cancer of the Colon

Ashley J. Snider, Agnieszka B.
Bialkowska, Amr M. Ghaleb, Vincent W. Yang, Lina M. Obeid, and Yusuf A. Hannun

15. Mouse Models for Studying
Depression-Like States and Antidepressant Drugs

Carisa L. Bergner, Amanda N.
Smolinsky, Peter C. Hart, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte,
and Allan V. Kalueff

16. Experimental Models of Anxiety for
Drug Discovery and Brain Research

Peter C. Hart, Carisa L. Bergner,
Amanda N. Smolinsky, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte, and
Allan V. Kalueff

<17. Repetitive Behavioral Assessments
for Compound Screening in Mouse Models of Autism Spectrum Disorders

Stacey J. Sukoff Rizzo

18. Better Utilization of Mouse Models
of Neurodegenerative Diseases in Preclinical Studies: From the Bench to the
Clinic

Christopher Janus, Carolina
Hernandez, Victoria deLelys, Hanno Roder, and Hans Welzl

19. Neuromuscular Disease Models and
Analysis

Robert W. Burgess, Gregory A. Cox,
and Kevin L. Seburn

20. Brightfield Imaging and Optical Coherence Tomography of the Mouse
Posterior Eye

Mark
P. Krebs, Mei Xiao, Keith Sheppard, Wanda Hicks, and Patsy M. Nishina

21. Mouse
Models as Tools to Identify Genetic Pathways for Retinal Degeneration, as
Exemplified by Leber's Congenital Amaurosis

Bo
Chang