Main description:

This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research. Recent genome studies, together with refined genetic engineering techniques, have greatly increased the value of using mice for research on cancer and other human disorders. The chapters of this book will support scientists in choosing the most suitable mouse models for their research questions. The book provides detailed methodological information for genetic or chemical induction of different types of cancer, histomorphometric cancer analysis, and in vivo imaging, as well as protocols to investigate oncogene addiction, immune surveillance, and hallmarks of cancer such as angiogenesis or metastasis. Four review-like articles provide background information on mouse technologies and histopathologic differences between mouse and human cancers. The mouse models described in individual chapters will fuel the understanding of cancer initiation, immune system roles, tumor angiogenesis, invasion, metastasis, and the relevance of molecular diversity observed among human cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and resourceful, Mouse Models of Cancers: Methods and Protocols, is a valuable laboratory resource for all researchers, from the graduate level upwards, who study cancer and new possibilities for its treatment.

Contents:

Part I Background
1. Modeling Cancer Using Genetically Engineered Mice
Patricia Stiedl, Beatrice Grabner, Katalin Zboray,
Edith Bogner, and Emilio Casanova

2. Lung Adenocarcinomas: Comparison Between Mice and Men
Helmut H. Popper

Part II Individual Cancers
3. Mouse Models of Breast Cancer
Kazuhito Sakamoto, Jeffrey W. Schmidt, and Kay-Uwe Wagner

4. Genetically Engineered Mouse Models to Study Prostate
Cancer
Elspeth A. Brzezinska, Colin Nixon, Rachana Patel, and Hing Y. Leung

5. Practical Use of Advanced Mouse Models for Lung Cancer
Roghaiyeh Safari and Ralph Meuwissen

6. Generation and Analysis of Mouse Intestinal Tumors and Organoids Harboring APC and K-Ras Mutations
Johan H. van Es and Hans Clevers

7. Induction of Colorectal Cancer in Mice and Histomorphometric Evaluation of Tumors
Illija Crncec, Paulina Pathria, Jasmin Svinka, and Robert Eferl

8. Mouse Models of Liver Cancer
Jorge Matias Caviglia and Robert F. Schwabe

9. Current Methods in Mouse Models of Pancreatic Cancer
Pawel K. Mazur, Alexander Herner, Florian Neff, and Jens T. Siveke

10. Mouse Models of Non-Melanoma Skin Cancer
Nicole Amberg, Martin Holcmann, Elisabeth Glitzner, Philipp Novoszel, Gabriel Stulnig, and Maria Sibilia

11. Clinicopathological Characterization of Mouse Models of Melanoma
Blake Ferguson, H. Peter Soyer, and Graeme J. Walker

12. Modeling BCR/ABL Driven Malignancies in the Mouse
Christine Schneckenleithner, Andrea Hoelbl-Kovacic, and Veronika Sexl

13. Methods to Generate Genetically-Engineered Mouse Models of Soft-Tissue Sarcoma
Rebecca D. Dodd, Leonor Ano, Jordan M. Blum, Zhizhong Li, David Van Mater, and David G. Kirsch

14. Characterization of Mouse Model-derived Osteosarcoma (OS) Cells in vitro and in vivo
OEzge Uluckan, Latifa Bakiri, and Erwin F. Wagner

15. Genetically Engineered Mouse and Orthotopic Human Tumor Xenograft Models of Retinoblastoma
Claudia A. Benavente and Michael A. Dyer

Part III Specific Aspects
16. Tumor Imaging Technologies in Mouse Models
Michael Bouvet and Robert M. Hoffman

17. Tumor Angiogenesis: Methods to Analyze Tumor Vasculature and Vessel Normalization in Mouse Models of Cancers
Federica Maione and Enrico Giraudo

18. Transplantable Mouse Tumor Models of Breast Cancer Metastasis
Rumela Chakrabarti and Yibin Kang

19. Methods to Study Tumor Cells and Residual Tumor Cells in Mouse Models of Oncogene Dependence
Caroline Botta, Cedric Darini, Guillaume Darrasse-Jeze, and Katrina Podsypanina

20. Generation of Transgenic Mouse Model Using PTTG as an Oncogene
Sham S. Kakar and Cohin Kakar

21. Modeling the Study of DNA Damage Responses in Mice
Julia Specks, Maria Nieto-Soler, Andres J. Lopez-Contreras, and Oscar Fernandez-Capetillo

22. Methods to Study Tumor Surveillance Using Tumor Cell Transplantation into Genetically Engineered Mice
Eva Bauer, Agnieszka Witalisz, Birgit Strobl, and Dagmar Stoiber