Wisepress Medical Bookshop

MORE ABOUT THIS BOOK

Main description:

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.

Contents:

1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins

Karine Rousseau and Dallas M. Swallow

2. Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies

Julia R. Davies, Claes Wickstroem and David J. Thornton

3. Detecting, Visualising and Quantifying Mucins

Ceri A. Harrop, David J. Thornton and Michael A. McGuckin

4. Mass Spectrometric Analysis of Mucin Core Proteins

Mehmet Kesimer and John K. Sheehan

5. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

Thomas A. Gerken

6. Analysis of Assembly of Secreted Mucins

Malin E.V. Johansson and Gunnar C. Hansson

7. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes

Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey

8.Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides

Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunseng Jin, and Niclas G. Karlsson

9. O-Glycomics: Profoliling and Structural Analysis of Mucin-type O-linked Glycans

Isabelle Breloy

10. O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry

Franz-Georg Hanisch

11. Analysing Mucin Degradation

Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield

12. Assessment of Mucus Thickness and Production In Situ

Lena Holm and Mia Phillipson

13. Preservation of Mucus in Histrological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH

Malin E.V. Johansson and Gunnar C. Hansson

14. In vitro Measurements of Mucus Secretion by Colon Explants

Jenny K. Gustafsson, Henrik Sjoevall and Gunnar C. Hansson

15. Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function

David R. Hill and Brian Button

16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis

17. Assessment of Intracellular Mucin Content In Vivo

Lucia Piccotti, Burton F. Dickey, Christopher M. Evans

18. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

Yong H. Sheng, Sumaira Z. Hasain, Chin Wen Png, Michael A. McGuckin and Sara K. Linden

19. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro

Pablo Augueso and Ilene K. Gipson