Main description:

This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer-based approaches to study muscarinic receptors. Muscarinic Receptor: From Structure to Animal Models guides readers through binding techniques, protocols to investigate molecular properties of muscarinic receptors, protocols to study muscarinic receptors in the central nervous system using autoradiography and PET studies and protocols on animals with knock-out and knock-in muscarinic genes to study role of muscarinic receptors in physiology and behavior. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Muscarinic Receptor: From Structure to Animal Models aims to ensure successful results in the further study of this vital field.

Contents:

1. Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors

Ryoji Suno, Hidetsugu Asada, and Takuya Kobayashi

2. Homology Modeling and Docking Evaluation of Human Muscarinic Acetylcholine Receptors

Trayder Thomas, David K. Chalmers, and Elizabeth Yuriev

3. Radioligand Binding at Muscarinic Receptors

Esam E. El-Fakahany and Jan Jakubik

4. Binding Method for Detection of Muscarinic Acetylcholine Receptors in Receptor's Natural Environment

Ikunobu Muramatsu, Hatsumi Yoshiki, Kiyonao Sada, Junsuke Uwada, Takanobu Taniguchi, Takayoshi Masuoka, and Matomo Nishio

5. Use of Antibodies in the Research on Muscarinic Receptor Subtypes Wisuit Pradidarcheep and Martin C. Michel

6. Allosteric Modulation of Muscarinic Receptors

Jan Jakubik and Esam E. El-Fakahany

7. Subcellular and Synaptic Localization of Muscarinic Receptors in Neurons Using High-Resolution Electron Microscopic Preembedding Immunogold Technique

Veronique Bernard

8. Investigation of Muscarinic Receptors by Fluorescent Techniques

Cornelius Krasel, Andreas Rinne, and Moritz Bunemann

9. Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System

Vladimir Farar and Jaromir Myslivecek

10. Imaging of Muscarinic Receptors in the Central Nervous System

Hideo Tsukada, Shingo Nishiyama, and Kazuhiro Takahashi

11. Detection of Non-Neuronal Acetylcholine

Ignaz Karl Wessler and Charles James Kirkpatrick

12. Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release

Vladimir Dolezal and Esam E. El-Fakahany

13. Regulation of Heart Contractility by M2 and M3 Muscarinic Receptors: Functional Studies using Muscarinic Receptor Knockout Mouse

Takio Kitazawa, Hiroki Teraoka, Nao Harada, Kenta Ochi, Tatsuro Nakamura, Koichi Asakawa, Shinya Kanegae, Noriko Yaosaka, Toshihiro Unno, Sei-ichi Komori, and Masahisa Yamada

14. Muscarinic Receptor Gene Transfections and In Vivo Dopamine Electrochemistry: Muscarinic Receptor Control of Dopamine-Dependent Reward and Locomotion

Stephan Steidl, David Ian Wasserman, Charles D. Blaha, and John Yeomans