Main description:

The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.

Contents:

Part I. Making Bio-Nano Interfaces

1. Preparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications

Daniel F. Moyano, Bradley Duncan, and Vincent M. Rotello

2. DNA Conjugation to Nanoparticles

Sunho Park

3. Conjugation of Nanoparticles to Proteins

Marie-Eve Aubin-Tam

4. Water-Solubilization and Functionalization of Semiconductor Quantum Dots

Christina M. Tyrakowski, Adela Isovic, and Preston T. Snee

5. Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates

W. Russ Algar, Juan B. Blanco-Canosa, Rachel L. Manthe, Kimihiro Susumu, Michael H. Stewart, Philip E. Dawson, and Igor L. Medintz

6. Reliable Methods for Silica Coating of Au Nanoparticles

Isabel Pastoriza-Santos and Luis M. Liz-Marzan

7. Surface Modifications by Polymers for Biomolecule Conjugation

Laura Sola, Marina Cretich, Francesco Damin, and Marcella Chiari

8. Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors

Franscesca Frascella and Carlo Ricciardi

Part II. Characterizing Bio-Nano Interfaces

9. Stability and Aggregation Assays of Nanoparticles in Biological Media

James Kah

10. Electrochemical Measurements of DNA Melting on Surfaces

Irina Belozerova, Dongbiao Ge, and Rastislav Levickyy

11. Formation and Characterization of Nanoparticle-Protein Corona

Marco P. Monopoli, Andrzej S. Pitek, Iseult Lynch, and Kenneth A. Dawson

12. Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution

Hyeran Noh, Naris Barnthip, Purnendu Parhi, and Erwin A. Vogler

13. Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment

Christin Grabinski, John Schlager, and Saber Hussain

14. Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles

Roberto Marotta, A. Falqui, A. Curcio, A. Quarta, and Teresa Pellegrino

Part III. Implementing Bio-Nano Interfaces

15. Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption

Zaki G. Estephan and Joseph B. Schlenoff

16. Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles

Xi-Jun Chen, Robert J. Hickey, and So-Jung Park

17. Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging

Taku Cowger and Jin Xie

18. Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy

Daniel Montiel, Emma V. Yates, Li Sun, Marissa M. Sampias, John Malona, Eirk J. Sorensen, and Haw Yang

19. Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs

Tennyson Doane and Clemens Burda

20. Modification of Carbon Nanotubes for Gene Delivery Vectors

Victor Ramos-Perez, Anna Cifuentes, Nuria Coronas, Ana de Pablo, and Salvador Borros

21. Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors

Daniele Pezzoli, Anna Kajaste-Rudnitski, Roberto Chiesa, and Gabriele Candiani

22. Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation

Joan Comenge and Victor F. Puntes