Main description:

Nanotechnology plays a key leading role in developing tools able to identify, measure, and study cellular events at the nanometric level as well as in contributing to the disclosure of unknown biological interactions and mechanisms, which opens the door for advances including nanodevices for diagnostic and therapy, drug delivery systems, and regenerative medicine. In Nanotechnology in Regenerative Medicine: Methods and Protocols, expert researchers in the field provide an overview of a very wide range of currently used technologies and methods that involve nanotechnology principles applicable to tissue regeneration. Being that the application of nanotechnology to regenerative medicine is a very broad field, this book focuses its interests on particular areas such as its use as a means to produce efficient platforms and structures for tissue engineering, delivery systems and biosensors, as well as the use of some techniques to study materials surfaces and the interactions between cells, biomolecules, and surfaces at the nanoscale. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their related topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Nanotechnology in Regenerative Medicine: Methods and Protocols provides established scientists, junior researchers, and students involved in the bioengineering, biotechnology, and biomedical fields with a sound foundation in a variety of vital nanotechnology approaches in regenerative medicine.

Contents:

1. Is Nanotechnology the Key to Unravel and Engineer Biological Processes?

Melba Navarro and Josep A. Planell

2. Synthesis of Genetically Engineered Protein Polymers (Recombinamers) as an Example of Advanced Self-Assembled Smart Materials

Jose Carlos Rodriguez-Cabello, Alessandra Girotti, Artur Ribeiro, and Francisco Javier Arias

3. Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine

Alvaro Mata, Liam Palmer, Esther Tejeda-Montes, and Samuel I. Stupp

4. Stimuli Responsive Polymers for Nanoengineering of Biointerfaces

Szczepan Zapotoczny

5. Micro-/Nano-Patterning of Proteins Using a Nanoimprint-Based Contact Printing Technique

Elena Martinez, Mateu Pla-Roca, and Josep Samitier

6. Functionalization of Surfaces with Synthetic Oligonucleotides

Brendan Manning and Ramon Eritja

7. Fabrication of PEG Hydrogel Microwell Arrays for High-Throughput Single Stem Cell Culture and Analysis

Stefan A. Kobel and Matthias P. Lutolf

8. Preparation of Polyelectrolyte Nanocomplexes Containing Recombinant Human Hepatocyte Growth Factor (rhHGF) as Potential Oral Carriers for Liver Regeneration

Catarina Ribeiro, Ana Patricia Neto, Jose das Neves, Maria Fernanda Bahia, and Bruno Sarmento

9. Electrospinning Technology in Tissue Regeneration

Oscar Castano, Mohamed Eltohamy, and Hae-Won Kim

10. Protein Adsorption Characterization

M. Cristina L. Martins, Susana R. Sousa, Joana C. Antunes, and Mario A. Barbosa

11. Measuring Wettability of Biosurfaces at the Microscale

Conrado Aparicio, Yassine Maazouz, and Dehua Yang

12. AFM to Study Bio/Non-Bio Interactions

Holger Schoenherr

13. Evaluation of Cytocompatibility and Cell Response to Boron Nitride Nanotubes

Gianni Ciofani and Serena Danti

14. Nanobiosensors for In Vitro and In Vivo Analysis of Biomolecules

J.-Pablo Salvador, Mark P. Kreuzer, Romain Quidant, Goncal Badenes, and M.-Pilar Marco

15. Novel Strategies to Engineering Biological Tissue In Vitro

Francesco Urciuolo, Giorgia Imparato, Angela Guaccio, Benedetto Mele, and Paolo A. Netti

16. Enabling Biomedical Research with Designer Quantum Dots

Nikodem Tomczak, Dominik Janczewski, Denis Dorokhin, Ming-Yong Han, and G. Julius Vancso

17. The Role of Nanophotonics in Regenerative Medicine

Maria F. Garcia-Parajo

18. Molecular Dynamics Methods for Modeling Complex Interactions in Biomaterials

Antonio Tilocca

19. Regenerative Nanomedicine: Ethical, Legal, and Social Issues

Linda MacDonald Glenn and Jeanann S. Boyce