Main description:

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural antibodies from human plasma, saliva, breast milk, and gastrointestinal fluids; functional properties of natural antibodies such as anti-tumor cytotoxic activity, and hydrolysis and dissolution of their target antigens; their utility in serological diagnosis of microbial antigens; and the role of natural antibodies in inhibiting viral vectors in the absence of prior exposure to the virus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Natural Antibodies: Methods and Protocols is a valuable resource for researchers and experts who are interested in further studies of natural antibodies.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Natural Antibodies: Next Steps towards Translational Investigation
Heinz Kohler

2. Isolation of Natural Anti-FceRIa Autoantibodies from Healthy Donors Monique Vogel, and Michael P. Horn

Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid
Christoph Schneider, Marlies Illi, Marius Loetscher, Marc Wehrli, and Stephan von Gunten

Purification of Natural Antibodies against Tau Protein by Affinity Chromatography
Michala Kolarova, Lenka Hromadkova, and Jan Ricny

Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires
Hanane El Bannoudi, Celine Anquetil, Marc Braunstein, Sergei L. Kosakovsky Pond, and Gregg J. Silverman

Analysis of Signaling Events in B-1a Cells
Jianxin Huo, Yuhan Huang, Shengli Xu, and Kong-Peng Lam

Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally-Secreted Immunoglobulins
Elodie Mohr and Andreia C. Lino

Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma
Reinhard Schwartz-Albiez and Othmar Dill

Hydrolysis and Dissolution of Amyloids by Catabodies
Ville V. Meretoja, Sudhir Paul, and Stephanie A. Planque

Methods for Post-Translational Induction of Polyreactivity of Antibodies
Maxime Lecerf, Annaelle Jarossay, Srinivas V. Kaveri, Sebastien Lacroix- Desmazes, and Jordan D. Dimitrov

Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles
Florian Puhm and Christoph J. Binder

Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans
Chunguang Wang and Sohvi Hoerkkoe

Detection of Natural Antibodies and Serological Diagnosis of Pneumococcal Pneumonia using a Bead-Based High-Throughput Assay
Irene Jimenez-Munguia, Willem J.B. van Wamel, Manuel J. Rodriguez-Ortega, and Ignacio Obando

Detection of Naturally Occurring Human Antibodies against Gangliosides by ELISA
Ana Maria Hernandez and Nely Rodriguez-Zhurbenko

Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors
Zhili Xu, Jie Tian, Andrew W. Harmon, and Andrew P. Byrnes