Main description:

With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum. Fully updating and adding to the previous two editions, Natural Products Isolation, Third Edition documents the latest methods and technologies for natural products isolation with a combination of all new chapters and revised and expanded classic methods. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Natural Products Isolation, Third Edition provides the substantial background information needed by budding natural product researchers as well offering an invaluable reference guide to available methodologies and techniques for the more experienced researchers.

Contents:

1. An Introduction to Natural Products Isolation

Satyajit D. Sarker and Lutfun Nahar

2. Initial and Bulk Extraction of Natural Products Isolation

Veronique Seidel

3. Supercritical Fluid Extraction in Natural Products Analyses

Lutfun Nahar and Satyajit D. Sarker

4. Accelerated Solvent Extraction (ASE (R)) for Natural Products Isolation

Mohammad A. Mottaleb and Satyajit D. Sarker

5. Microwave-Assisted Extraction in Natural Products Isolation

Abbas Delazar, Lutfun Nahar, Sanaz Hamedeyazdan, and Satyajit D. Sarker

6. An Introduction to Planar Chromatography and Its Application to Natural Products Isolation

Simon Gibbons

7. Isolation of Natural Products by Low-Pressure Column Chromatography

Raymond G. Reid and Satyajit D. Sarker

8. Isolation of Natural Products by Ion-Exchange Methods

Klaus Dragull and John J. Beck

9. Separation of Natural Products by Countercurrent Chromatography

James B. McAlpine, J. Brent Friesen, and Guido F. Pauli

10. Isolation of Natural Products by Preparative High Performance Liquid Chromatography (Prep-HPLC)

Zahid Latif and Satyajit D. Sarker

11. Isolation of Natural Products by Preparative Gas Chromatography

Temel OEzek and Fatih Demirci

12. Hyphenated Techniques and Their Applications in Natural Products Analysis

Satyajit D. Sarker and Lutfun Nahar

13. Extraction of Plant Secondary Metabolites

William P. Jones and A. Douglas Kinghorn

14. Isolation of Marine Natural Products

Wael E. Houssen and Marcel Jaspars

15. Isolation of Microbial Natural Products

Olov Sterner

16. Extraction and Isolation of Saponins

Runner R.T. Majinda

17. Extraction and Isolation of Phenolic Compounds

Celestino Santos-Buelga, Susana Gonzalez-Manzano, Montserrat Duenas, and Ana M. Gonzalez-Paramas

18. Scaling-Up of Natural Products Isolation

Yash Kumarasamy

19. Follow-Up of Natural Products Isolation

Richard J.P. Cannell, Satyajit D. Sarker, and Lutfun Nahar

20. Natural Products Isolation in Modern Drug Discovery Programs

Alexander I. Gray, John O. Igoli, and RuAngelie Edrada-Ebel