Main description:

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP- expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.

Contents:

Obituary...Preface to the Series...Preface...Table of Contents...Contributing Authors...
1 Neurohistology with a Touch of History: Technology-Driven ResearchFloris G. Wouterlood and Thomas P. Langer
2 Fixation Protocols for Neurohistology: Neurons to Genes Elliott J. Mufson, Sylvia E. Perez, Christy M. Kelley, Melissa J. Alldred, and Stephen D. Ginsberg
3 Three-Dimensional Atlases of Insect BrainsBasil el Jundi and Stanley Heinze
4 Neuroanatomical Tracing Based on Selective Fluorochrome Expression in Transgenic animalsFloris G. Wouterlood
5 Optical Imaging Probes for Amyloid Diseases in BrainPratyush Kumar Mishra, Myeong-Gyun Kang, Hyun-Woo Rhee
6 Chemical Clearing of GFP-Expressing Neural TissuesKlaus Becker, Saiedeh Saghafi, Christian Hahn, Nina Jahrling, and Hans Ulrich Dodt
7 The Properties of Light Governing Biological MicroscopyPina Colarusso
8 Beyond Brightfield: "Forgotten" Microscopic ModalitiesRadek Pelc
9 Stereomicroscopy in NeuroanatomyErin E. Wilson, William Chambers, Radek Pelc, Paul Nothnagle, and Michael W. Davidson
10 Conventional, Apodized, and Relief Phase-Contrast MicroscopyRadek Pelc, Zdenek Hostounsky, Tatsuro Otaki, and Kaoru Katoh
11 Ultramicroscopy of Nerve Fibers and Neurons: Fine-Tuning the Light SheetsSaiedeh Saghafi, Klaus Becker, Nina Jahrling, Christian Hahn, and Hans Ulrich Dodt
12 Imaging and Electrophysiology of Individual Neurites Functionally Isolated in MicrochannelsHeinz D. Wanzenboeck, Petra Scholze, and Johann K. Mika
13 Consumer Versus Dedicated Digital Cameras in PhotomicrographyJoerg Piper and Radek Pelc
14 Digital Micrographs in PathologyRoger S. Riley, Jorge Almenara, and Christine E. Fuller
Subject Index List ...