Main description:

The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced undergraduates who seek an overview of methods and protocols in this field of neuroscience. It will also be of interest to physicians who are carrying out imaging and postmortem studies of neurotransmitter transporters in the human brain. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the
further study of this vital field.

Contents:

Part I: Recent Improvements
in LV Construction, Production, and Transduction

1. Construction of Modular Lentiviral Vectors for Effective Gene
Expression and Knockdown

Angeline De Bruyns, Ben Geilin, and David Dankort

2. Development of Inducible Molecular Switches Based on All-In-One
Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase

Tobias Maetzig and Axel
Schambach

3.
Production Of Retrovirus-Based
Vectors In Mildly Acidic Ph Conditions

Nathalie Holic And David Fenard

4. Optimized Lentiviral
Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and
Scfv-Antibody Fusions To VSV-G

Natasa Anastasov, Ines Hoefig, Sabine Mall, Angela M. Krackhardt,
and Christian Thirion

Part II: New LV Targets
and Applications

5.
Transduction of Murine
Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors

Maike Stahlhut, Axel Schambach, and Olga
S. Kustikova

6. Introduction of Shrnas, Mirnas or Antagomirs into
Primary Human Liver Cells Through Lentiviral Vectors

Jessica
K. Rieger and Maria Thomas

7. Production and Concentration of Lentivirus for Transduction of Primary
Human T Cells

Alan Kennedy and Adam P. Cribbs

8. Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa
Followed by In Vitro Fertilization and Embryo Transfer

Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston

9. The LAM-PCR Method to Sequence LV Integration Sites

Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette
Deichmann, and Manfred Schmidt

10. Conditional Rnai using the Lentiviral GLTR System

Elisabeth Pfeiffenberger and Stephan Geley

11. Lentiviral Vectors for the Engineering of Implantable Cells Secreting
Recombinant Antibodies

Aurelien Lathuiliere and Bernard L. Schneider

III. Integrase-Mutant
Lvs

12. Transient Expression of Green Fluorescent Protein in Integrase-Defective
Lentiviral Vector Transduced 293T Cell Line

Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin
Farzaneh, and MK Azaham A Hamid

13. Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a
Rat Model of Parkinson's Disease

Ngoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. Yanez-Munoz

14. Development of Lentiviral Vectors for Targeted Integration and Protein
Delivery

Diana Schenkwein and Seppo Yla-Herttuala

Part IV: Production,
Detection, and Engineering of Exosomes

15. Biogenesis and Functions of Exosomes and Extracellular Vesicles

Florian Dreyer and
Andreas Baur

16. Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes

Carolina
Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana
Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo

17. Cardiac Myocyte Exosome Isolation

Zulfiqar A. Malik and Anne A. Knowlton

18.
Incorporation
of Heterologous Proteins in Engineered Exosomes

Francesco Manfredi, Paola Di
Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico

19. Exosome-Mediated Targeted Delivery of
Mirnas

Shin-Ichiro
Ohno and Masahiko Kuroda