Main description:

This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Pig Cloning Using Somatic Cell Nuclear Transfer

Hongsheng Ouyang, Jianyong Han, and Yongye Huang

2. Cloning of Monkeys by Somatic Cell Nuclear Transfer Shiyu Sun, Zhaodi Liao, Zhen Liu, and Qiang Sun

3. Production of Cardiomyocyte-like cells by Fibroblast Reprogramming with Defined Factors

Emre Bektik and Ji-dong Fu

4. Production of Cardiomyocytes by microRNA-mediated Reprogramming In Optimized Reprogramming Media

Xiaowen Wang, Conrad P Hodgkinson, and Victor J Dzau

5. Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-free Cells

Minhyung Lee, Janghwan Kim, and Rajesh Ambasudhan

6. Generation of Human Neurons by microRNA-mediated Direct Conversion of Dermal Fibroblasts

Victoria A. Church, Kitra Cates, Lucia Capano, Shivani Aryal, Woo Kyung Kim, and Andrew S. Yoo

7. Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-marked Lentiviral Vectors in the Chemically Defined Medium

Zhicheng Shao, Ricardo Cevallos, and Kejin Hu

8. Episomal Reprogramming Of Human Peripheral Blood Mononuclear Cells Into Pluripotency

Wei Wen, Tao Cheng, and Xiao-Bing Zhang

9. Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells

Fabia Febbraro, Muwan Chen, and Mark Denham

10. Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling

Chun Liu, Mohamed Ameen, Sukaina Himmati, Dilip Thomas, and Nazish Sayed

11. Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA Robert R. Annand

12. Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators

Jere Weltner, and Ras Trokovic

13. Reprogramming Porcine Fibroblast to EPSCs

Xuefei Gao, Degong Ruan, and Pentao Liu

14. Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance

Jennifer L. Brehm, Molly M. Miles, Katie S. Remondini, and Tenneille E. Ludwig

15. Evaluate Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers

Ricardo R. Cevallos, Md Emon Hossain, Ruowen Zhang, and Kejin Hu

16. G-banded Karyotyping of Human Pluripotent Stem Cell Cultures

Erik McIntire, Kimberly Leonhard, Seth Taapken, and Anna Lisa Larson

17. Become Competent in Generating RNA-seq Heat Maps in One Day for Novices without prior R Experience

Kejin Hu