Main description:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Contents:

Genome mining and sequence analysis of chemosensory soluble proteins in arthropods
Joel Vizueta, Paula Escuer, Alejandro Sanchez-Gracia and Julio Rozas

Reconstructing protein coding sequences from ancient DNA
Michael Hofreiter and Stefanie Hartmann

Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs
Jean-Francois Picimbon

Proteomics of arthropod soluble olfactory proteins
Francesca R. Dani and Giuseppe Pieraccini

Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects
Andrea Scaloni

Bacterial expression and purification of vertebrate odorant-binding proteins
Marine Brule, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loic Briand

Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
Anais Gaubert, Beatrice Amigues, Silvia Spinelli and Christian Cambillau

Solution structure of insect CSP and OBPs by NMR
Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone

Stability of OBPs
Nadja Hellmann

Ligand-binding assays with OBPs and CSPs
Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi

Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins
Rudolf Alexander Steinbrecht

Effect of OBPs on the response of olfactory receptors
Bing Wang, Song Cao, Wei Liu and Guirong Wang

Site-directed mutagenesis of odorant-binding proteins
Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi

Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
Yusuke Shiota and Takeshi Sakurai

Electrochemical biosensing with odorant binding proteins
Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu

Electronic biosensors based on graphene FETs
Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll

Electronic biosensors based on EGOFETs
Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli

Gravimetric biosensors
Khasim Cali, Elena Tuccori and Krishna C. Persaud

Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins

Anil Bozdogan, Simone Hageneder and Jakub Dostalek