Main description:

The advancements of medical technology, improvements in medical care, and increased patients' life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner. Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Protocols, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Pain Research: Methods and Protocols, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models.

Contents:

1. Advancements in Pain Research

Z. David Luo

2. Genomic Methods for Clinical and Translational Pain Research

Dan Wang, Hyungsuk Kim, Xiao-Min Wang, and Raymond Dionne

3. Two-Dimensional Gel Electrophoresis: Discovering Neuropathic Pain-Associated Synaptic Biomarkers in Spinal Cord Dorsal Horn

Om V. Singh and Yuan-Xiang Tao

4. Whole-Cell Patch-Clamp Recordings on Spinal Cord Slices

Ping Deng and Zao C. Xu

5. Whole-Cell Recording in Isolated Primary Sensory Neurons

Michael S. Gold

6. Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing

Julie Wieseler, David Sprunger, Amanda Ellis, Steven F. Maier and Linda R. Watkins

7. An Experimental Model of Headache-Related Pain

Rebecca M. Edelmayer, Michael H. Ossipov and Frank Porreca

8. A Rodent Model of Trigeminal Neuralgia

David C. Yeomans and Mikhail Klukinov

9. New Models of Experimental Parotitis and Parotid Gland Distension in Rats

Akio Okada-Ogawa, Masamichi Shinoda, Kuniya Honda, and Koichi Iwata

10. A Rat Pain Model of Facial Cancer

Kentaro Ono, Nozomu Harano, Kiyotoshi Inenaga, and Osamu Nakanishi

11. Orofacial Pain Models and Behavior Assessment

Timothy K. Y. Kaan, Peter T. Ohara, and Luc Jasmin

12. Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain

Julie Wieseler, Amanda Ellis, Steven F. Maier, Linda R. Watkins, and Scott Falci

13. A Lumbosacral Ventral Root Avulsion Injury and Repair Model for Studies of Neuropathic Pain in Rats

Leif A. Havton

14. A Rat Chronic Pain Model of Spinal Cord Contusion Injury

Kelli Sharp, Amin Boroujerdi, Oswald Steward, and Z. David Luo

15. The Spared Nerve Injury Model of Neuropathic Pain

Marie Pertin, Romain-Daniel Gosselin, and Isabelle Decosterd

16. A New Rat Pain Model of Thrombus-Induced Ischemia

Jang-Hern Lee

17. Rat Models of Pancreatitis Pain

Karin Westlund High and Louis Vera-Portocarrero

18. The Monosodium Iodoacetate Model of Osteoarthritis Pain in The Rat

Cheryl L. Marker and James D. Pomonis

19. K/BxN Serum Transfer Arthritis as a Model of Inflammatory Joint Pain

Christina Christianson, Maripat Corr, Tony L. Yaksh, and Camilla I. Svensson

20. A New Rat Model of Bone Cancer Pain

Ruixin Zhang and Lixing Lao

21. Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury

Danielle Perret, Doo-Sik Kim, Kang-Wu Li, and Z. David Luo