Main description:

Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA. Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes. The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.

Contents:

1. Identification and Analysis of Precursors to Invasive Pancreatic Cancer

Hanno Matthaei, Marco Dal Molin, Anirban Maitra,

2. Tissue Microarrays: Construction and Use

Helen Remotti

3. Immunohistochemistry of Pancreatic Neoplasia

Sukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar and

Surinder K. Batra

4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer

Lorenzo F. Sempere, and Murray Korc

5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications

Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova

6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis

Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue

7. DNA Methylation Analysis in Human Cancer

Eileen O'Sullivan, Michael Goggins

8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients

Michael J. Baine, Kavita Mallya, and Surinder K. Batra

9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee

10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach

Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht

11. Development of Orthotopic Pancreatic Tumor Mouse Models

Wanglong Qiu, Gloria H. Su

12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems

Emman Mascarinas, Guido Eibl , Paul J. Grippo

13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound

Stephen A. Sastra and Kenneth P. Olive

14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells

Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi

15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach

16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis

Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang

17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo

Chen Huang and Keping Xie

18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells

Richard D. Dinnen, Yuehua Mao, and Robert L. Fine

19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods

Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra

20. Evaluating Tumor Angiogenesis

Minji Kim, Jessica Kandel, Jan Kitajewski

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer

Lorenzo F. Sempere, and Murray Korc

5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications

Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova

6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis

Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue

7. DNA Methylation Analysis in Human Cancer

Eileen O'Sullivan, Michael Goggins

8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients

Michael J. Baine, Kavita Mallya, and Surinder K. Batra

9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee

10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach

Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht

11. Development of Orthotopic Pancreatic Tumor Mouse Models

Wanglong Qiu, Gloria H. Su

12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems

Emman Mascarinas, Guido Eibl , Paul J. Grippo

13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound

Stephen A. Sastra and Kenneth P. Olive

14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells

Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi

15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach

16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis

Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang

17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo

Chen Huang and Keping Xie

18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells

Richard D. Dinnen, Yuehua Mao, and Robert L. Fine

19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods

Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra

20. Evaluating Tumor Angiogenesis

Minji Kim, Jessica Kandel, Jan Kitajewski

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee

10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach

Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht

11. Development of Orthotopic Pancreatic Tumor Mouse Models

Wanglong Qiu, Gloria H. Su

12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems

Emman Mascarinas, Guido Eibl , Paul J. Grippo

13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound

Stephen A. Sastra and Kenneth P. Olive

14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells

Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi

15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach

16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis

Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang

17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo

Chen Huang and Keping Xie

18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells

Richard D. Dinnen, Yuehua Mao, and Robert L. Fine

19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods

Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra

20. Evaluating Tumor Angiogenesis

Minji Kim, Jessica Kandel, Jan Kitajewski

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells

Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach

16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis

Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang

17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo

Chen Huang and Keping Xie

18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells

Richard D. Dinnen, Yuehua Mao, and Robert L. Fine

19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods

Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra

20. Evaluating Tumor Angiogenesis

Minji Kim, Jessica Kandel, Jan Kitajewski

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

20. Evaluating Tumor Angiogenesis

Minji Kim, Jessica Kandel, Jan Kitajewski

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

21. Pooled Genome-wide RNAi Screenings

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva

22. Pooled ShRNA screenings: Computational Analysis

Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva