Main description:

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Contents:

1. Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes

Reidar Andreson, Lauris Kaplinski, and Maido Remm

2. Primer Design for PCR Reactions in Forensic Biology

Kelly M. Elkins

3. Design of Primers and Probes For Quantitative Real-Time PCR MethodsAlicia Rodriguez, Mar Rodriguez, Juan J. Cordoba, and Maria J. Andrade

4. Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A VirusNagy Alexander, Jirinec, Tomas, Jirincova, Helena, and Havlickova, Martina

5. Low-concentration Initiator Primers Improve The

Amplification Of Gene Targets With High Sequence Variability

Kenneth E. Pierce and Lawrence J. Wangh

6. Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens

Wenchao Yan

7. Degenerate Primer Design for Highly Variable Genomes

Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell

8. Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection

Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu

9. MultiPLX: Automatic Grouping And Evaluation Of PCR Primers

Lauris Kaplinski and Maido Remm

10. In silico PCR Primer Designing and Validation

Anil Kumar and Nikita Chordia

11. Primer Design using Primer Express (R) for SYBR Green Based Quantitative PCR

Amarjeet Singh and Girdhar K. Pandey

12. Designing Primers for SNaPshot Technique

Greiciane Gaburro Paneto and Francisco de Paula Careta

13. Rapid and Simple Method of qPCR Primer Design

Brenda Thornton and Chhandak Basu

14. PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design

Garima Kushwaha

15. Selecting Specific PCR Primers with MFEprimerWubin Qu and Chenggang Zhang