Main description:

This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.

Contents:

1. Solution Phase Peptide Synthesis: The Case of Biphalin

Dagmara Tymecka and Aleksandra Misicka

2. Application of Fmoc-SPPS, Thiol-Maleimide Conjugation and Copper(I)-Catalyzed Alkyne-Azide Cycloaddition "Click" Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate against Group A Streptococcus

Chuankai Dai, Rachel J. Stephenson, Mariusz Skwarczynski, and Istvan Toth

3. In Situ Neutralization Protocols for Boc-SPPS

Ramkrishna Adhikary and Philip E. Dawson

4. Anhydrous Hydrogen Fluoride Cleavage in Boc Solid Phase Peptide Synthesis

Kirtikumar B. Jadhav, Katrina J. Woolcock, and Markus Muttenthaler

5. Automated Solid-Phase Peptide Synthesis

Dirk F.H. Winkler

6. New Developments in Microwave-Assisted Solid Phase Peptide Synthesis

Sandeep K. Singh and Jonathan M. Collins

7. Protecting Groups in Peptide Synthesis

Maddeboina Krishnaiah and Martin Conda-Sheridan

8. Synthesis of O-Acyl Isopeptides: Stepwise and Convergent Solid-Phase Synthesis

Taku Yoshiya

9. An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides

Waleed M. Hussein, Mariusz Skwarczynski, and Istvan Toth

10. SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces

Dirk F.H. Winkler

11. Solid-Phase Synthesis of Wollamide Cyclohexapeptide Analogs

Allan M. Prior and Dianqing Sun

12. Synthesis of Branched Peptides via a Side-Chain Benzyl Ester

Junjie Liu, Jian Li, Xiaobo Tian, Feng Tang, and Wei Huang

13. Solid Phase Synthesis of Octapeptin Lipopeptides

Karl A. Hansford, Zyta M. Ziora, Matthew A. Cooper, and Mark A.T. Blaskovich

14. Peptidomimetic Synthesis Drug Discovery for Alzheimer's Disease

Yoshio Hamada and Zyta M. Ziora

15. Synthesis of Amide Backbone-Modified Peptides

Abu-Baker M. Abdel-Aal, Richard Raz, George Papageorgiou, and John Offer

16. Synthesis of NickFects, a New Family of CPPs, by Solid-Phase Peptide Synthesis

Piret Arukuusk and UElo Langel

17. Chemoenzymatic Synthesis of HIV-1 Glycopeptide Antigens

Guanghui Zong, Chao Li, and Lai-Xi Wang

18. Synthesis of Antimicrobial Lipopeptides Using the "CLipPA" Thiol-Ene Reaction

Yann O. Hermant, Alan J. Cameron, Paul W.R. Harris, and Margaret A. Brimble

19. Decarboxylative Couplings for Late-Stage Peptide Modifications

Meng Yao Zhang and Lara R. Malins

20. Peptide and Pseudopeptide Bond Synthesis in Phosphorus Dipeptide Analogs

Artur Mucha and Pawel Kafarski

21. Micro-Bioreactor Techniques for the Production and Spectroscopic Characterization of Microbial Peptides

Zeinab G. Khalil, Angela A. Salim, and Robert J. Capon

22. Metal Peptide Complexes for Study Neurodegenerative Diseases

Mariusz Mital, Jakub P. Sek, and Zyta M. Ziora