Main description:

Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Contents:

1. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells

Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko

2. Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo

Sidney B. Cambridge

3. Optochemical Activation of Kinase Function in Live Cells

Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters

4. Photoswitching of Cell Surface Receptors Using Tethered Ligands

Andreas Reiner and Ehud Y. Isacoff

5. Photocontrol of AMPA Receptors with a Photochromic Ligand

Martin Sumser and Philipp Stawski

6. Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy

Nina Wittenmayer

7. Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins

Lauren R. Polstein and Charles A. Gersbach

8. Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions

Olof Idevall-Hagren and Pietro DeCamilli

9. A Roadmap to Applying Optogenetics in Neuroscience

Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh

10. Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors

Marion Mutter, Natalia Swietek & Thomas A. Munch

11. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity

Wagner Steuer Costa, Jana Liewald, Alexander Gottschalk

12. Structural Basis of Photoswitching in Fluorescent Proteins

Chenxi Duan, Virgile Adam, Martin Byrdin and Dominique Bourgeois

13. Using Photoactivatable GFP to Track Axonal Transport Kinetics

Archan Ganguly and Subhojit Roy

14. In Vivo Cell Tracking Using PhOTO Zebrafish

William P. Dempsey, Hanyu Qin, Periklis Pantazis

15. In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein

Cathleen Teh and Vladimir Korzh

16. Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy

Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus

17. pcSOFI as a Smart Label-based Super resolution Microscopy Technique

Benjamien Moeyaert and Peter Dedecker