Main description:

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Contents:

Part I: Metabolites and Metabolic Fluxes

1. MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism

Catherine Deborde and Daniel Jacob

2. Targeted Deuteration of Polyphenolics for their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts

Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman

3. Relative Quantitation in Single Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry

Bindesh Shrestha and Akos Vertes

4. Quantification of Plant Volatiles

Anthony V. Qualley and Natalia Dudareva

5. Quantitative Imaging Approaches for Small Molecule Measurements using FRET Sensors in Plants

Sakiko Okumoto

6. Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance

Quyen X. Truong, Jong Moon Yoon, and Jacqueline V. Shanks

7. Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry

Jamey D. Young, Douglas K. Allen, and John A. Morgan

8. Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux AnalysisShilpa Nargund and Ganesh Sriram

9. Optimal Design of Isotope Labeling Experiments

Hong Yang, Dominic E Mandy and Igor GL Libourel

Part II: Metabolic Pathways and Metabolic Systems Biology

10. Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities

Kate Dreher

11. Elucidation of Metabolic Pathways from Enzyme Classification Data

Andrew G. McDonald and Keith F. Tipton

12. Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data

Ansgar Gruber and Peter G. Kroth

13. Genome-scale Models of Plant MetabolismMargaret Simons, Ashish Misra, and Ganesh Sriram

14. Elementary Flux Modes, Flux Balance Analysis and their Application to Plant MetabolismKatrin Lotz, Anja Hartmann, Eva Grafahrend-Belau, Falk Schreiber, and Bjoern H. Junker

15. Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways

Martin J. Spiering, Bhavneet Kaur, James F. Parsons, and Edward Eisenstein

16. Applications of Kinetic Modeling to

Plant Metabolism

Johann M. Rohwer

17. Kinetic Modeling of Plant Metabolism and its Predictive Power - Peppermint Essential Oil Biosynthesis as an Example

Bernd Markus Lange and Rigoberto Rios-Estepa