Main description:

With numerous methods available for the quantification of
nitric oxide (NO), this detailed book explores their advantages and
disadvantages in order to provide a foundation for further research in plant
nitric oxide. After providing a useful practical guide to choosing a technique
for measuring NO from plant materials, the book continues with chapters on chemiluminescence,
diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as
many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory methods, and tips on troubleshooting
and avoiding known pitfalls.

Practical and authoritative, Plant
Nitric Oxide: Methods and Protocols serves as a valuable guide to all
researchers working and intending to work in the field of plant nitric oxide
research.

Contents:

1. An Overview of
Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple
Methods?

Hideo
Yamasaki, Naoko S. Watanabe, Yasuko Sakihama, and Michael F. Cohen

2. Chemiluminescence Detection of
Nitric Oxide from Roots, Leaves, and Root Mitochondria

Aakanksha Wany, Alok K .
Gupta, , Aprajita Kumari, Shika Gupta,
Sonal Mishra, Ritika Jaintu, Pradeep K. Phatak, and Kapuganti Jagadis Gupta

3. Nitric
Oxide Measurement from Purified Enzymes and Estimation of Scavenging Activity
by Gas Phase Chemlimunimescence Method

Aprajita Kumari, Alok K.
Gupta, Sonal Mishra, Aakanksha Wany, and Kapuganti Jagadis Gupta

4. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence

Aakanksha Wany and
Kapuganti Jagadis Gupta

5. Nitric Oxide (NO) Measurements in
Stomatal Guard Cells

Srinivas Agurla, Gunja
Gayatri, and Agepati S. Raghavendra

6. Methods to Detect Nitric Oxide in Plants: Are DAFs
Really Measuring NO?

Stefan Ruemer, Markus
Krischke, Agnes Fekete, Maria Lesch, Marin J. Mueller, and Werner M. Kaiser

7. A
Novel Protocol for Detection of Nitric Oxide in Plants

Prachi Jain and Satish
C. Bhatla

8. Detection of Nitric Oxide by Electron Paramagnetic
Resonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates

Luisa B. Maia and Jose
J.G. Moura

9. Measurement of Nitric Oxide (NO)
Generation Rate by Chloroplasts Employing Electron Spin Resonance (ESR)

Andrea Galatro and Susana Puntarulo

10. Laser-Based Methods for Detection of Nitric Oxide in
Plants

Julien Mandon, Luis A.J. Mur, Frans
J.M. Harren, and Simona M. Cristescu

11. Electrochemical Detection of Nitric Oxide in Plant
Cell Suspensions

Sophie Griveau,
Angelique Besson-Bard, Fethi Bedioui, and David Wendehenne

12. Quantification and Localization of S-Nitrosothiols (SNOs) in Higher Plants

Juan B. Barroso, Raquel
Valderrama, Alfonso Carreras, Mounira Chaki, Juan C. Begara-Morales, Beatriz
Sanchez-Calvo, and Francisco J. Corpas

13. Analysis of the Expression and Activity
of Nitric Oxide Synthase from Marine Photosynthetic Microorganisms

Foresi Noelia, Correa
Aragunde Natalia, Santolini Jerome, and Lamattina Lorenzo

14. Identification
of S-Nitrosothiols by the Sequential
Cysteine Blocking Technique

Rafael A. Homem, Thierry
Le Bihan, Manda Yu, and Gary J. Loake

15. Detection
of S-Nitrosoglutathione Reductase Activity in Plants

Lucie Kubienova, Tereza
Ticha, Lenka Luhova, and Marek Petrivalsky

16. Detection
of Peroxynitrite in Plants Exposed to Bacterial Infection

Diana Bellin, Massimo
Delledonne, and Elodie Vandelle