Main description:

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.

Contents:

1. Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method

Hanako Shimura, Kazuyoshi Furuta, and Chikara Masuta

2. RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers

Tyler D. B. MacKenzie, Xianzhou Nie, and Mathuresh Singh

3. New Method to Isolate Total dsRNA

Go Atsumi, Ken-Taro Sekine, and Kappei Kobayashi

4. Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses

Giorgio Gambino

5. Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

Takahide Sasaya

6. Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector

Emanuela Noris and Laura Miozzi

7. Detection and Analysis of Non-Retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes

Hideki Kondo, Sotaro Chiba and Nobuhiro Suzuki

8. Detection of Plant Viruses in Natural Environments by Using RNA-Seq

Atsushi J. Nagano, Mie N. Honjo, Motohiro Mihara, Masanao Sato, and Hiroshi Kudoh

9. Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA

Yuan-Yuan Fang, Neil A. Smith, Jian-Hua Zhao, Joanne R. M. Lee, Hui-Shan Guo, and Ming-Bo Wang

10. Drawing siRNAs of Viral Origin out from Plant siRNAs Libraries

Laura Miozzi and Vitantonio Pantaleo

11. Viral Detection by High-Throughput Sequencing

Daisuke Motooka, Shota Nakamura, Katsuro Hagiwara, and Takaaki Nakaya

12. Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing

Charith Raj Adkar-Purushothama, Zhixiang Zhang, Shifang Li, and Teruo Sano

13. Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing

Tatsuhiro Ezawa, Yoji Ikeda, Hanako Shimura, and Chikara Masuta

14. SuperSAGE as an Analytical Tool for Host and Viral Gene Expression

Hideo Matsumura, Detlev H. Kruger, Gunter Kahl, and Ryohei Terauchi

15. Microarray Analysis of R-Gene-Mediated Resistance to Viruses

Takeaki Ishihara, Yukiyo Sato, and Hideki Takahashi

16. Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus

Kenji S. Nakahara, Kei Nishino, and Ichiro Uyeda

17. Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors

Chunjiang Li and Nobuyuki Yoshikawa

18. Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones

Tatsuya Nagata and Alice Kazuko Inoue-Nagata

19. Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control

Andreas E. Voloudakis, Maria C. Holeva, Peter L. Sarin, Dennis H. Bamford, Marisol Vargas, Minna M. Poranen, and Francisco Tenllado

20. Detection of Plant Virus in Meristem by Immunohistochemistry and In Situ Hybridization

Tomofumi Mochizuki and Satoshi T. Ohki